Male-killing Wolbachia and mitochondrial selective sweep in a migratory African insect.
Male-killing Wolbachia and mitochondrial selective sweep in a migratory African insect.
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DOI：
10.1186/1471-2148-12-204
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发表时间：
2012-10-15
影响因子：
3.4
通讯作者：
Wilson K
中科院分区：
文献类型：
--
作者：
Graham RI;Wilson K
近年来的大量研究表明，昆虫的共生微生物在宿主生态学和进化中起着重要作用。鳞翅目害虫非洲粘虫（Spodoptera examputa）是一种高度洄游和破坏性的物种，遍布撒哈拉以南非洲，可在一代人的时间内爆发，使得预测种群动态和害虫防治预测极为困难。最近在坦桑尼亚的采样人群中发现了三种沃尔巴克氏体感染该物种。在这项研究中，我们研究了Wolbachia pipiensis感染和共同遗传标记，mtDNA，粘虫种群内的相互作用，作为一种手段，调查的种群生物学和进化历史的Wolbachia及其主机。在实验室建立了一个Wolbachia感染的同雌系。表型研究证实菌株wExe 1是一种雄性杀手。部分测序的线粒体COI基因从164个人现场收集的粘虫已知的感染状态显示17个不同的单倍型。Wolbachia感染状态和mtDNA单倍型之间有很强的相关性，单倍型1（90.2%的患病率）是一个单一的优势单倍型，窝藏内共生体。所有三种沃尔巴克氏体菌株都与该单倍型相关。这表明，沃尔巴克氏体可能是驱动粘虫单倍型多样性的选择性扫描。尽管有非常强的生物学和分子证据表明这些样本代表了一个单一的物种（包括来自核28 S基因标记），但这17个单倍型并不属于灰翅夜蛾属内的单系分支;其中6个单倍型（2个来自3个地理上独立的种群）与其他11个单倍型的核苷酸序列差异>11%。这项研究表明，三个菌株的沃尔巴克氏体可能是驱动选择性扫描粘虫单倍型多样性，并根据COI序列数据，S。在灰翅夜蛾属中，无翅夜蛾不是一个单系群。这对mtDNA作为昆虫中性遗传标记的使用具有明确的意义，也表明了Wolbachia感染对宿主进化遗传学的影响。
Numerous recent studies have shown that resident symbiotic microorganisms of insects play a fundamental role in host ecology and evolution. The lepidopteran pest, African armyworm (Spodoptera exempta), is a highly migratory and destructive species found throughout sub-Saharan Africa, that can experience eruptive outbreaks within the space of a single generation, making predicting population dynamics and pest control forecasting extremely difficult. Three strains of Wolbachia have recently been identified infecting this species in populations sampled from Tanzania. In this study, we examined the interaction between Wolbachia pipiensis infections and the co-inherited marker, mtDNA, within populations of armyworm, as a means to investigate the population biology and evolutionary history of Wolbachia and its host. A Wolbachia-infected isofemale line was established in the laboratory. Phenotypic studies confirmed the strain wExe1 as a male-killer. Partial sequencing of the mitochondrial COI gene from 164 individual field-collected armyworm of known infection status revealed 17 different haplotypes. There was a strong association between Wolbachia infection status and mtDNA haplotype, with a single dominant haplotype, haplo1 (90.2% prevalence), harbouring the endosymbiont. All three Wolbachia strains were associated with this haplotype. This indicates that Wolbachia may be driving a selective sweep on armyworm haplotype diversity. Despite very strong biological and molecular evidence that the samples represent a single species (including from nuclear 28S gene markers), the 17 haplotypes did not fall into a monophyletic clade within the Spodoptera genus; with six haplotypes (2 each from 3 geographically separate populations) differing by >11% in their nucleotide sequence to the other eleven. This study suggests that three strains of Wolbachia may be driving a selective sweep on armyworm haplotype diversity, and that based on COI sequence data, S. exempta is not a monophyletic group within the Spodoptera genus. This has clear implications for the use of mtDNA as neutral genetic markers in insects, and also demonstrates the impact of Wolbachia infections on host evolutionary genetics.
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DOI：
10.1098/rsbl.2003.0025
发表时间：
2003-08-07
影响因子：
4.7
作者：
Hebert, PDN;Ratnasingham, S;deWaard, JR
通讯作者：
deWaard, JR
影响因子：
3.8
作者：
Dyson, EA;Kamath, MK;Hurst, GDD
通讯作者：
Hurst, GDD
DOI：
10.1073/pnas.101304298
发表时间：
2001-05-22
影响因子：
11.1
作者：
Dedeine, F;Vavre, F;Boulétreau, M
通讯作者：
Boulétreau, M
影响因子：
3.4
作者：
Charlat S;Duplouy A;Hornett EA;Dyson EA;Davies N;Roderick GK;Wedell N;Hurst GD
通讯作者：
Hurst GD
影响因子：
16.8
作者：
Fenn, K;Blaxter, M
通讯作者：
Blaxter, M