Neurochemical coding of enteric neurons in adult and embryonic zebrafish (Danio rerio).
Neurochemical coding of enteric neurons in adult and embryonic zebrafish (Danio rerio).
复制标题
DOI：
10.1002/cne.22464
复制
发表时间：
2010-11-01
影响因子：
2.5
通讯作者：
Van Nassauw, Luc
中科院分区：
文献类型：
--
作者：
Uyttebroek, Leen;Shepherd, Iain T.;Harrisson, Fernand;Hubens, Guy;Blust, Ronny;Timmermans, Jean-Pierre;Van Nassauw, Luc
虽然斑马鱼肠神经系统的形态和发育已被广泛研究，肠神经元的精确神经化学编码及其比例肠分布目前尚不清楚。通过免疫组织化学方法，我们确定了神经化学标记物在胚胎和成年斑马鱼肠道中的比例表达和共表达。酪氨酸羟化酶（TH），血管活性肠肽（VIP），垂体腺苷酸环化酶激活肽（PACAP）只在神经纤维中观察到，而其他标志物也检测到神经元细胞体。钙视网膜蛋白和钙结合蛋白的分布相似。在胚胎中，所有的标记，除了胆碱乙酰转移酶（ChAT）和TH，存在从受精后72小时。氮能神经元，均匀分布，并保持恒定的时间，构成了主要的神经元亚群。其他标记物的神经元比例在发育过程中增加，其特征在于区域差异。在成人中，所有检测的标志物均在肠神经系统中表达。大部分肠神经元显示钙结合蛋白和钙视黄蛋白，血清素是唯一的标记物，显示在三个肠区域的显着分布差异。共定位研究表明，血清素不与任何其他标志物共表达。至少有五个神经元亚群进行了测定：胆碱能，氮能noncholinergic，两个胆碱能nonnitrergic亚群沿着一个亚群表达ChAT和神经元型一氧化氮合酶。神经纤维的分析表明，氮能神经元共表达VIP和PACAP，并且氮能神经元支配图尼卡肌层，而胆碱能和胆碱能非氮能神经元支配固有层和图尼卡肌层。
Although the morphology and development of the zebrafish enteric nervous system have been extensively studied, the precise neurochemical coding of enteric neurons and their proportional enteric distribution are currently not known. By using immunohistochemistry, we determined the proportional expression and coexpression of neurochemical markers in the embryonic and adult zebrafish intestine. Tyrosine hydroxylase (TH), vasoactive intestinal peptide (VIP), and pituitary adenylate cyclase-activating peptide (PACAP) were observed only in nerve fibers, whereas other markers were also detected in neuronal cell bodies. Calretinin and calbindin had similar distributions. In embryos, all markers, except for choline acetyltransferase (ChAT) and TH, were present from 72 hours postfertilization. Nitrergic neurons, evenly distributed and remaining constant in time, constituted the major neuronal subpopulation. The neuronal proportions of the other markers increased during development and were characterized by regional differences. In the adult, all markers examined were expressed in the enteric nervous system. A large percentage of enteric neurons displayed calbindin and calretinin, and serotonin was the only marker showing significant distribution differences in the three intestinal regions. Colocalization studies showed that serotonin was not coexpressed with any of the other markers. At least five neuronal subpopulations were determined: a serotonergic, a nitrergic noncholinergic, two cholinergic nonnitrergic subpopulations along with one subpopulation expressing both ChAT and neuronal nitric oxide synthase. Analysis of nerve fibers revealed that nitrergic neurons coexpress VIP and PACAP, and that nitrergic neurons innervate the tunica muscularis, whereas serotonergic and cholinergic nonnitrergic neurons innervate the lamina propria and the tunica muscularis.
登录
查看更多内容
影响因子：
2.5
作者：
GOODRICH, JT;BERND, P;GERSHON, MD
通讯作者：
GERSHON, MD
影响因子：
3.6
作者：
Dénes, V;Gábriel, R
通讯作者：
Gábriel, R
影响因子：
3.5
作者：
Burzynski G;Shepherd IT;Enomoto H
通讯作者：
Enomoto H
影响因子：
3.6
作者：
BELAI, A;COOPER, S;BURNSTOCK, G
通讯作者：
BURNSTOCK, G
影响因子：
2.2
作者：
Fornaro, Michele;Raimondo, Stefania;Giacobini-Robecchi, Maria Giuseppina
通讯作者：
Giacobini-Robecchi, Maria Giuseppina