Increased prevalence of anellovirus in pediatric patients with fever.
Increased prevalence of anellovirus in pediatric patients with fever.
复制标题
DOI：
10.1371/journal.pone.0050937
复制
发表时间：
2012
期刊：
影响因子：
3.7
通讯作者：
Storch GA
中科院分区：
文献类型：
--
作者：
McElvania TeKippe E;Wylie KM;Deych E;Sodergren E;Weinstock G;Storch GA
Anelloviridae家族由无包膜、环状、单链DNA病毒组成。已知感染人类的病毒有三个属，分别是TTV、TTMDV和TTMV。虽然最初认为非甲-庚型病毒性肝炎是由甲型肝炎病毒引起的，但到目前为止，持续的研究表明，甲型肝炎病毒与人类疾病之间没有明确的联系。利用高通量测序，我们调查了2-36个月大的儿科患者中贫血病毒与发热之间的关系。我们确定，尽管在发热和无热患者的大量标本中都存在阿涅罗病毒，但与无热对照相比，它们在发热患者的血浆和鼻咽(NP)标本中更普遍。用聚合酶链式反应对感染人类的三种病毒进行检测，发现发热患者血浆和NP中的病毒种类TTV和TTMDV比发热对照更普遍。但TTMV的情况并非如此，在发热和无热的患者标本中发现了类似比例的TTMV。对患者年龄的分析表明，血浆和NP标本中含有病毒的百分比随着年龄的增长而增加，直到19-24个月龄时，病毒阳性的患者标本的百分比下降。在TTV和TTMDV中，这一趋势是显著的，而在血浆和NP标本中，TTMV的趋势非常轻微。最后，随着发热患者体温的升高，TTV和TTMDV的检出频率也随之增加。同样，TTMV同样存在于发热和无热的患者样本中。综合这些数据，表明人类病毒TTV和TTMDV与儿童发烧有关，而高度相关的人类病毒TTMV与发烧无关。
The Anelloviridae family consists of non-enveloped, circular, single-stranded DNA viruses. Three genera of anellovirus are known to infect humans, named TTV, TTMDV, and TTMV. Although anelloviruses were initially thought to cause non-A-G viral hepatitis, continued research has shown no definitive associations between anellovirus and human disease to date. Using high-throughput sequencing, we investigated the association between anelloviruses and fever in pediatric patients 2–36 months of age. We determined that although anelloviruses were present in a large number of specimens from both febrile and afebrile patients, they were more prevalent in the plasma and nasopharyngeal (NP) specimens of febrile patients compared to afebrile controls. Using PCR to detect each of the three species of anellovirus that infect humans, we found that anellovirus species TTV and TTMDV were more prevalent in the plasma and NP specimens of febrile patients compared to afebrile controls. This was not the case for species TTMV which was found in similar percentages of febrile and afebrile patient specimens. Analysis of patient age showed that the percentage of plasma and NP specimens containing anellovirus increased with age until patients were 19–24 months of age, after which the percentage of anellovirus positive patient specimens dropped. This trend was striking for TTV and TTMDV and very modest for TTMV in both plasma and NP specimens. Finally, as the temperature of febrile patients increased, so too did the frequency of TTV and TTMDV detection. Again, TTMV was equally present in both febrile and afebrile patient specimens. Taken together these data indicate that the human anellovirus species TTV and TTMDV are associated with fever in children, while the highly related human anellovirus TTMV has no association with fever.
登录
查看更多内容
影响因子：
13.5
作者：
Kanda, T;Yokosuka, O;Saisho, H
通讯作者：
Saisho, H
影响因子：
3.8
作者：
Biagini, Philippe;Uch, Rathviro;de Micco, Philippe
通讯作者：
de Micco, Philippe
影响因子：
5.4
作者：
Miyata, H;Tsunoda, H;Hino, S
通讯作者：
Hino, S
影响因子：
9.4
作者：
Hsieh, SY;Wu, YH;Liaw, YF
通讯作者：
Liaw, YF
影响因子：
2.2
作者：
Goto, K;Sugiyama, K;Wada, Y
通讯作者：
Wada, Y