Strengthening the skin with topical delivery of keratinocyte growth factor-1 using a novel DNA plasmid.
Strengthening the skin with topical delivery of keratinocyte growth factor-1 using a novel DNA plasmid.
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DOI：
10.1038/mt.2014.2
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发表时间：
2014-04
影响因子：
12.4
通讯作者：
Harmon, John W.
中科院分区：
文献类型：
--
作者：
Dou, Chunqing;Lay, Frank;Ansari, Amir Mehdi;Rees, Donald J.;Ahmed, Ali Karim;Kovbasnjuk, Olga;Matsangos, Aerielle E.;Du, Junkai;Hosseini, Sayed Mohammad;Steenbergen, Charles;Fox-Talbot, Karen;Tabor, Aaron T.;Williams, James A.;Liu, Lixin;Marti, Guy P.;Harmon, John W.
脆弱的皮肤，容易受到褥疮和附带创伤，是一个问题，特别是对老年人和那些有脊髓损伤。在这里，我们提出了一种简单的方法，通过局部递送角质细胞生长因子-1 (KGF-1) DNA来增强皮肤。在初步可行性研究中，利用新型的最小化、无抗生素DNA表达载体NTC8385-VA1，传递了报告基因荧光素酶和增强型绿色荧光蛋白。转染后荧光素酶表达显著增加。增强的绿色荧光蛋白转染皮肤的显微镜成像显示，毛囊、毛轴、真皮和浅表上皮细胞都有绿色荧光。转染KGF-1后，分别用定量逆转录-聚合酶链反应和免疫组织化学方法记录KGF-1 mRNA水平和蛋白产量。转染后48小时，KGF组皮肤上皮细胞厚度较对照组显著增加（26±2µm比16±4µm） （P = 0.045）。KGF组皮肤厚度在120小时有增加的趋势（255±36 vs 162±16µm） （P = 0.057）。生物力学评估显示，kgf -1处理的皮肤明显强于对照载体转染的皮肤。这些发现表明，局部递送KGF-1 DNA质粒可以增加上皮细胞的厚度和强度，证明了这种方法修复受损皮肤的潜力。
Fragile skin, susceptible to decubitus ulcers and incidental trauma, is a problem particularly for the elderly and for those with spinal cord injury. Here, we present a simple approach to strengthen the skin by the topical delivery of keratinocyte growth factor-1 (KGF-1) DNA. In initial feasibility studies with the novel minimalized, antibiotic-free DNA expression vector, NTC8385-VA1, the reporter genes luciferase and enhanced green fluorescent protein were delivered. Transfection was documented when luciferase expression significantly increased after transfection. Microscopic imaging of enhanced green fluorescent protein–transfected skin showed green fluorescence in hair follicles, hair shafts, and dermal and superficial epithelial cells. With KGF-1 transfection, KGF-1 mRNA level and protein production were documented with quantitative reverse transcriptase–polymerase chain reaction and immunohistochemistry, respectively. Epithelial thickness of the transfected skin in the KGF group was significantly increased compared with the control vector group (26 ± 2 versus 16 ± 4 µm) at 48 hours (P = 0.045). Dermal thickness tended to be increased in the KGF group (255 ± 36 versus 162 ± 16 µm) at 120 hours (P = 0.057). Biomechanical assessment showed that the KGF-1–treated skin was significantly stronger than control vector–transfected skin. These findings indicate that topically delivered KGF-1 DNA plasmid can increase epithelial thickness and strength, demonstrating the potential of this approach to restore compromised skin.
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影响因子：
2.4
作者：
Lee, Woan-Ruoh;Tsai, Ren-Yeu;Fang, Jia-You
通讯作者：
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