Evaluation of Dittrichia viscosa Aquaporin Nip1.1 Gene as Marker for Arsenic-Tolerant Plant Selection.
Evaluation of Dittrichia viscosa Aquaporin Nip1.1 Gene as Marker for Arsenic-Tolerant Plant Selection.
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DOI：
10.3390/plants11151968
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发表时间：
2022-07-28
期刊：
影响因子：
4.5
通讯作者：
Di Sansebastiano, Gian-Pietro
中科院分区：
文献类型：
--
作者：
De Paolis, Angelo;De Caroli, Monica;Rojas, Makarena;Curci, Lorenzo Maria;Piro, Gabriella;Di Sansebastiano, Gian-Pietro
关键词：
粘性双毛藻Dittrichiasvsa(L.)Greuter因其高度的遗传可塑性和适应恶劣环境条件的能力而受到关注，包括重金属和金属污染。镉、铜、铁、镍、铅和锌的吸收和转运已经被表征，但它在砷的作用下的表现却鲜为人知，有时甚至相互矛盾。拟南芥对砷的耐受性与吸收减少无关，但水通道蛋白Nip1.1基因的零突变使植物对类金属具有完全的抗性。这种水通道蛋白定位于内质网，负责亚砷酸盐和锑(Sb)膜的渗透，但亚砷酸盐的摄取也发生在缺失突变体中，提示有比直接摄取更复杂的作用机制。本研究克隆了DvNip1基因同源物，并对其在不同砷胁迫条件下根和地上部的表达谱进行了研究。克隆系的使用可以证明DvNip1.1的表达水平受到砷胁迫的影响。根和地上部的基因表达比例可以用来产生一个指数，该指数似乎是一个很有前途的选择标记，可以用来预测粘性毛滴虫的抗砷品系。
Dittrichia viscosa (L.) Greuter is gaining attention for its high genetic plasticity and ability to adapt to adverse environmental conditions, including heavy metal and metalloid pollution. Uptake and translocation of cadmium, copper, iron, nickel, lead, and zinc to the shoots have been characterized, but its performance with arsenic is less known and sometimes contradictory. Tolerance to As is not related to a reduced uptake, but the null mutation of the aquaporin Nip1.1 gene in Arabidopsis makes the plant completely resistant to the metalloid. This aquaporin, localized in the endoplasmic reticulum, is responsible for arsenite and antimony (Sb) membrane permeation, but the uptake of arsenite occurs also in the null mutant, suggesting a more sophisticated action mechanism than direct uptake. In this study, the DvNip1 gene homologue is cloned and its expression profile in roots and shoots is characterized in different arsenic stress conditions. The use of clonal lines allowed to evidence that DvNip1.1 expression level is influenced by arsenic stress. The proportion of gene expression in roots and shoots can be used to generate an index that appears to be a promising putative selection marker to predict arsenic-resistant lines of Dittrichia viscosa plants.
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影响因子：
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作者：
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