Sex-linked pheromone receptor genes of the European corn borer, Ostrinia nubilalis, are in tandem arrays.
Sex-linked pheromone receptor genes of the European corn borer, Ostrinia nubilalis, are in tandem arrays.
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DOI：
10.1371/journal.pone.0018843
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发表时间：
2011-04-22
期刊：
影响因子：
3.7
通讯作者：
Ishikawa Y
中科院分区：
文献类型：
--
作者：
Yasukochi Y;Miura N;Nakano R;Sahara K;Ishikawa Y
将雄蛾的嗅觉系统调整为同种雌蛾信息素对于正确的物种识别至关重要；然而，人们对这一系统中驱动物种形成的遗传变化知之甚少。Ostrinia属的飞蛾是解释这个问题的很好的模型，因为在物种内和物种之间观察到信息素混合的显著差异。气味受体(ORs)在识别雌虫性信息素方面起着关键作用，已有8种类型的OR基因在玉米蛾雄蛾触角中表达。本研究采用聚合酶链式反应(PCR)技术，筛选出一个亚洲玉米象细菌人工染色体(BAC)文库，并从含有已报道的OR基因的克隆中构建了3个重叠群。以这些克隆为探针的荧光原位杂交(FISH)分析表明，最大的重叠群位于Z染色体上，包含8个OR基因，另外2个包含2个和1个OR基因的常染色体上。对BAC克隆的测序结果表明，Z-连锁的OR基因紧密相连，排列有序，其中4个具有181个碱基的直接重复序列，跨越外显子7和内含子7。这是鳞翅目昆虫性信息素受体基因的首次报道。在Z染色体上的OR基因簇的定位与先前的Z连锁基因座的发现一致，该基因座负责对性信息素的雄性行为反应。181个碱基的直接重复序列可能通过不等交换增强基因复制。最近报道了一个常染色体上的基因座，该基因座至少含有4个OR基因，该基因座负责雄性对性信息素的反应。综上所述，这些发现支持这样的假设，即OR基因的额外拷贝的产生可以增加雄蛾获得信息素成分的改变的专一性的可能性，从而促进性信息素的分化。
Tuning of the olfactory system of male moths to conspecific female sex pheromones is crucial for correct species recognition; however, little is known about the genetic changes that drive speciation in this system. Moths of the genus Ostrinia are good models to elucidate this question, since significant differences in pheromone blends are observed within and among species. Odorant receptors (ORs) play a critical role in recognition of female sex pheromones; eight types of OR genes expressed in male antennae were previously reported in Ostrinia moths. We screened an O. nubilalis bacterial artificial chromosome (BAC) library by PCR, and constructed three contigs from isolated clones containing the reported OR genes. Fluorescence in situ hybridization (FISH) analysis using these clones as probes demonstrated that the largest contig, which contained eight OR genes, was located on the Z chromosome; two others harboring two and one OR genes were found on two autosomes. Sequence determination of BAC clones revealed the Z-linked OR genes were closely related and tandemly arrayed; moreover, four of them shared 181-bp direct repeats spanning exon 7 and intron 7. This is the first report of tandemly arrayed sex pheromone receptor genes in Lepidoptera. The localization of an OR gene cluster on the Z chromosome agrees with previous findings for a Z-linked locus responsible for O. nubilalis male behavioral response to sex pheromone. The 181-bp direct repeats might enhance gene duplications by unequal crossovers. An autosomal locus responsible for male response to sex pheromone in Heliothis virescens and H. subflexa was recently reported to contain at least four OR genes. Taken together, these findings support the hypothesis that generation of additional copies of OR genes can increase the potential for male moths to acquire altered specificity for pheromone components, and accordingly, facilitate differentiation of sex pheromones.
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DOI：
10.1017/cbo9780511542664.009
发表时间：
2004-01-01
期刊：
ADVANCES IN INSECT CHEMICAL ECOLOGY
影响因子：
--
作者：
Cardé, RT;Haynes, KF
通讯作者：
Haynes, KF
影响因子：
2.6
作者：
Bohbot, J.;Pitts, R. J.;Zwiebel, L. J.
通讯作者：
Zwiebel, L. J.
影响因子：
56.9
作者：
Nakagawa, T;Sakurai, T;Touhara, K
通讯作者：
Touhara, K
影响因子：
3.8
作者：
Engsontia, Patamarerk;Sanderson, Alan P.;Brown, Stephen
通讯作者：
Brown, Stephen
影响因子：
1.9
作者：
Ishikawa, Y;Takanashi, T;Huang, YP
通讯作者：
Huang, YP