Mechanism of nitric oxide enhancing NaCl tolerance of barley seedlings based on physiol-biochemical analysis and LC-MS metabolomics
Mechanism of nitric oxide enhancing NaCl tolerance of barley seedlings based on physiol-biochemical analysis and LC-MS metabolomics
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基于生理生化分析和LC-MS代谢组学的一氧化氮增强大麦幼苗耐氯化钠能力的机制
DOI：
10.1016/j.envexpbot.2021.104533
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发表时间：
2021-09
影响因子：
5.7
通讯作者：
Runqiang Yang
中科院分区：
文献类型：
--
作者：
Yan Ma;Yao Yi'an;Qiaoe Wang;Zhenxin Gu;Pei Wang;Yongbin Han;Runqiang Yang
•SNP减轻了60 mM NaCl对植株生长的抑制和氧化损伤。•0.2 mM snp处理的大麦幼苗表现出更高的抗氧化能力。•SNP促进了酚酸、异黄酮和花青素的生物合成。•SNP增强糖酵解、半乳糖、果糖和三羧酸代谢。一氧化氮（NO）参与了植物的非生物胁迫和大麦幼苗的耐盐性增强。然而，其内部机制尚不清楚。本研究通过研究NO及其特异性清除剂2,4-羧基苯基-4,4,5,5-四甲基咪唑啉-1-氧-3-氧化物（cPTIO）在NaCl胁迫下大麦幼苗的生理代谢组学特性，了解NO在耐NaCl胁迫中的关键作用。结果表明，硝普钠（SNP， NO供体）可减轻60 mM NaCl对生长的抑制和氧化损伤。0.2 mM SNP处理的大麦幼苗表现出较高的抗氧化酶活性、总酚含量、抗氧化能力和游离氨基酸含量。而0.5 mM的cPTIO则产生相反的效果。代谢组学分析结果发现，与NaCl胁迫相比，SNP增强了大麦幼苗糖酵解、半乳糖、果糖和甘露糖代谢，以及氨基酸生物合成和三羧酸代谢。综上所述，NO诱导大麦幼苗对NaCl胁迫的耐受性主要是通过促进酚类物质积累、增强抗氧化酶活性、增强抗氧化能力以及促进碳水化合物代谢和氨基酸的生物合成来实现的。
• SNP alleviated the growth inhibition and oxidative damage caused by 60 mM of NaCl. • The 0.2 mM SNP-treated barley seedlings exhibited higher antioxidant capacity. • SNP enhanced the biosynthesis of phenolic acids, isoflavones and anthocyanins. • SNP enhanced glycolysis, galactose, fructose and tricarboxylic acid metabolisms. Nitric oxide (NO) is involved in the abiotic stress of plants and enhanced NaCl tolerance of barley seedlings. However, the internal mechanism is unclear. In this study, the physiology and metabolomics characteristics of barley seedlings with the present of NO and its specific scavenger 2,4-carboxyphenyl-4,4,5,5-tetramethylimidazoline-1-oxyl-3-oxide (cPTIO) under NaCl stress were investigated to understand the key function of NO in NaCl tolerance. Results showed that sodium nitroprusside (SNP; a NO donor) alleviated the growth inhibition and oxidative damage caused by 60 mM of NaCl. The 0.2 mM SNP treated barley seedlings exhibited higher antioxidant enzymes activity, total phenolic content, antioxidant capacity, and free amino acids content. However, the 0.5 mM of cPTIO induced the opposite effects. Based on the results of metabolomics analysis, it was found that SNP enhanced glycolysis, galactose, fructose and mannose metabolism, as well as amino acids biosynthesis and tricarboxylic acid metabolisms in barley seedlings compared with NaCl stress. These results indicated that NO could induce barley seedlings tolerance to NaCl stress depending on its roles in enhancing phenolics accumulation, antioxidation enzymes activity, antioxidant capacity as well as accelerating the carbohydrate metabolism and amino acids biosynthesis.
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影响因子：
3.9
作者：
Oliveira, Halley C.;Gomes, Bruna C. R.;Seabra, Amedea B.
通讯作者：
Seabra, Amedea B.
影响因子：
2.9
作者：
PATTERSON, BD;MACRAE, EA;FERGUSON, IB
通讯作者：
FERGUSON, IB
影响因子：
5.6
作者：
Ma X;Xia H;Liu Y;Wei H;Zheng X;Song C;Chen L;Liu H;Luo L
通讯作者：
Luo L
硫化氢通过一氧化氮介导维持大麦幼苗根部离子稳态来增强耐盐性
影响因子：
1.6
作者：
Cheng, Jingyi;Wan, Zhijun;Zhang, Peng
通讯作者：
Zhang, Peng
DOI：
--
发表时间：
2011
期刊：
--
影响因子：
--
作者：
S. Zahedi;M. Nabipour;Mehrdad Azizi;H. Gheisary;M. Jalali;Z. Amini
通讯作者：
S. Zahedi;M. Nabipour;Mehrdad Azizi;H. Gheisary;M. Jalali;Z. Amini