A chemical window into the impact of RNAi silencing of the StNAC103 gene in potato tuber periderms: Soluble metabolites, suberized cell walls, and antibacterial defense.
A chemical window into the impact of RNAi silencing of the StNAC103 gene in potato tuber periderms: Soluble metabolites, suberized cell walls, and antibacterial defense.
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stnac103基因在马铃薯块茎periderms中的RNAi沉默的化学窗口：可溶性代谢产物，蛋白酶的细胞壁和抗菌防御。
DOI：
10.1016/j.phytochem.2021.112885
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发表时间：
2021-10
期刊：
影响因子：
3.8
通讯作者：
Stark RE
中科院分区：
文献类型：
--
作者：
Dastmalchi K;Chira O;Rodriguez MP;Yoo B;Serra O;Figueras M;Stark RE
陆生植物的生长和存活需要控制它们与环境的相互作用，例如，来抵御干燥和微生物的入侵。对于主要的粮食作物，外皮（马铃薯的外皮）所提供的保护对于可食用块茎和果实的种植、储存和销售是必不可少的。马铃薯特别容易受到细菌感染，因为它们的含水量高，易受机械伤害。最近，特异性和保守性基因沉默（StNAC 103-RNAi和StNAC 103-RNAi-c，分别）被发现增加负载的蜡和脂族软木解聚产物在块茎周皮，暗示这NAC基因作为一个阻遏物的蜡和软木生物合成途径。然而，我们对StNAC 103沉默的理解中的一个重要差距涉及在围壁细胞中产生的代谢物作为抗微生物防御剂和沉积的木栓素生物聚合物的潜在构建块。在目前的工作中，我们通过进行全面的平行分析来分析化学成分和抗菌活性的变化，扩展了对StNAC 103沉默线的先前研究。使用固态13 C NMR（ssNMR）的完整的木栓化细胞壁的组成分析表明，NAC沉默产生的长链脂肪族基团的增加，沉积在围壁细胞壁内。极性提取物的LC-MS显示StNAC 103-RNAi和StNAC 103-RNAi-c天然外表皮中糖苷生物碱的上调，但StNAC 103-RNAi-c中酚胺和StNAC 103-RNAi天然外表皮中酚酸的下调。使用GC-MS鉴定的非极性可溶性代谢物包括两种沉默样品中显著丰富的长链烷烃代谢物。通过协调差异积累的可溶性代谢产物和木栓素解聚产物与基于ssNMR的谱的peridendum聚合物，它是可能的，以获得一个整体的观点的化学变化，导致StNAC 103基因沉默。相应地，化学成分的趋势作为一个背景来解释天然块茎外表皮的化学屏障防御功能的趋势，这被认为是更强大的非极性提取物。
The growth and survival of terrestrial plants require control of their interactions with the environment, e.g., to defend against desiccation and microbial invasion. For major food crops, the protection conferred by the outer skins (periderm in potato) is essential to cultivation, storage, and marketing of the edible tubers and fruits. Potatoes are particularly vulnerable to bacterial infections due to their high content of water and susceptibility to mechanical wounding. Recently, both specific and conserved gene silencing (StNAC103-RNAi and StNAC103-RNAi-c, respectively) were found to increase the load of wax and aliphatic suberin depolymerization products in tuber periderm, implicating this NAC gene as a repressor of the wax and suberin biosynthetic pathways. However, an important gap in our understanding of StNAC103 silencing concerns the metabolites produced in periderm cells as antimicrobial defense agents and potential building blocks of the deposited suberin biopolymer. In the current work, we have expanded prior studies on StNAC103 silenced lines by conducting comprehensive parallel analyses to profile changes in chemical constituents and antibacterial activity. Compositional analysis of the intact suberized cell walls using solid-state 13C NMR (ssNMR) showed that NAC silencing produced an increase in the long-chain aliphatic groups deposited within the periderm cell walls. LC-MS of polar extracts revealed up-regulation of glycoalkaloids in both StNAC103-RNAi and StNAC103-RNAi-c native periderms but down-regulation of a phenolic amine in StNAC103-RNAi-c and a phenolic acid in StNAC103-RNAi native periderms. The nonpolar soluble metabolites identified using GC-MS included notably abundant long-chain alkane metabolites in both silenced samples. By coordinating the differentially accumulated soluble metabolites and the suberin depolymerization products with the ssNMR-based profiles for the periderm polymers, it was possible to obtain a holistic view of the chemical changes that result from StNAC103 gene silencing. Correspondingly, the chemical composition trends served as a backdrop to interpret trends in the chemical barrier defense function of native tuber periderms, which was found to be more robust for the nonpolar extracts.
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影响因子：
6.1
作者：
Dastmalchi K;Cai Q;Zhou K;Huang W;Serra O;Stark RE
通讯作者：
Stark RE
影响因子：
6.1
作者：
Dastmalchi K;Kallash L;Wang I;Phan VC;Huang W;Serra O;Stark RE
通讯作者：
Stark RE
影响因子：
3.8
作者：
Arrieta-Baez, D;Stark, RE
通讯作者：
Stark, RE
影响因子：
3.8
作者：
Dastmalchi, Keyvan;Rodriguez, Mathiu Perez;Stark, Ruth E.
通讯作者：
Stark, Ruth E.
影响因子：
8.8
作者：
Narvaez-Cuenca, Carlos-Eduardo;Vincken, Jean-Paul;Gruppen, Harry
通讯作者：
Gruppen, Harry