Prospects and challenges of CRISPR/Cas genome editing for the study and control of neglected vector-borne nematode diseases.
Prospects and challenges of CRISPR/Cas genome editing for the study and control of neglected vector-borne nematode diseases.
复制标题
DOI：
10.1111/febs.13781
复制
发表时间：
2016-09
期刊：
影响因子：
--
通讯作者：
Andersen EC
中科院分区：
文献类型：
--
作者：
Zamanian M;Andersen EC
由寄生线虫引起的被忽视的热带疾病给许多发展中国家造成了巨大的健康和社会经济负担。目前的估计表明，超过20亿人感染线虫，导致每年损失1400万残疾调整生命年。虽然这些寄生虫引起显著的死亡率，但它们主要通过广泛的严重临床疾病引起慢性发病率。线虫感染的治疗选择仅限于少数驱虫药，而驱虫药大规模给药的迅速扩大引起了对耐药性的担忧。有必要保留现有药物，并开发新的治疗选择和控制方法。我们将重点关注CRISPR/Cas9基因组编辑技术的民主化，以提高我们对线虫寄生虫生物学的理解以及治疗它们引起的感染的能力。我们将首先探讨如何使用这种强大的基因组操作方法来新开发强大的模式线虫寄生虫学研究。然后，我们将讨论在丝虫中开发CRISPR/Cas9编辑方案的潜在途径。最后，我们将提出CRISPR/Cas9可用于工程基因驱动的潜在方法，这些基因驱动靶向蚊媒丝虫的传播。
Neglected tropical diseases caused by parasitic nematodes inflict an immense health and socioeconomic burden throughout much of the developing world. Current estimates indicate that more than two billion people are infected with nematodes, resulting in the loss of 14 million disability‐adjusted life years per annum. Although these parasites cause significant mortality, they primarily cause chronic morbidity through a wide range of severe clinical ailments. Treatment options for nematode infections are restricted to a small number of anthelmintic drugs, and the rapid expansion of anthelmintic mass drug administration raises concerns of drug resistance. Preservation of existing drugs is necessary, as well as the development of new treatment options and methods of control. We focus this review on how the democratization of CRISPR/Cas9 genome editing technology can be enlisted to improve our understanding of the biology of nematode parasites and our ability to treat the infections they cause. We will first explore how this robust method of genome manipulation can be used to newly exploit the powerful model nematode Caenorhabditis elegans for parasitology research. We will then discuss potential avenues to develop CRISPR/Cas9 editing protocols in filarial nematodes. Lastly, we will propose potential ways in which CRISPR/Cas9 can be used to engineer gene drives that target the transmission of mosquito‐borne filarial nematodes.
登录
查看更多内容
影响因子：
30.8
作者：
Andersen, Erik C.;Gerke, Justin P.;Shapiro, Joshua A.;Crissman, Jonathan R.;Ghosh, Rajarshi;Bloom, Joshua S.;Felix, Marie-Anne;Kruglyak, Leonid
通讯作者：
Kruglyak, Leonid
影响因子：
46.9
作者：
通讯作者：
--
影响因子：
46.9
作者：
Cho, Seung Woo;Kim, Sojung;Kim, Jin-Soo
通讯作者：
Kim, Jin-Soo
影响因子：
1.9
作者：
Allen, ML;Christensen, BM
通讯作者：
Christensen, BM
影响因子：
3.5
作者：
Barnard AC;Nijhof AM;Fick W;Stutzer C;Maritz-Olivier C
通讯作者：
Maritz-Olivier C