Single-cell force spectroscopy of the medically important Staphylococcus epidermidis-Candida albicans interaction.
Single-cell force spectroscopy of the medically important Staphylococcus epidermidis-Candida albicans interaction.
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表皮葡萄球菌相互作用的医学重要葡萄球菌的单细胞力光谱。
DOI：
10.1039/c3nr03272h
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发表时间：
2013-11-21
期刊：
影响因子：
6.7
通讯作者：
Dufrêne YF
中科院分区：
文献类型：
--
作者：
Beaussart A;Herman P;El-Kirat-Chatel S;Lipke PN;Kucharíková S;Van Dijck P;Dufrêne YF
尽管细菌-真菌相互作用的临床重要性，其分子细节知之甚少。这种医学上重要的种间关联的一个标志是两种医院病原体金黄色葡萄球菌和白色念珠菌之间的相互作用，这可能导致混合生物膜相关感染，并增强抗生素耐药性。在这里，我们使用单细胞力谱来量化参与细菌-真菌共粘附的力，重点是研究不足的S。epidermidis-C.白色念珠菌相互作用单个细菌和真菌芽管之间记录的力曲线显示较大的粘附力（~5 nN），断裂长度延长（高达500 nm）。相比之下，细菌对酵母细胞的粘附较差，强调了酵母到菌丝的转变在介导对细菌细胞的粘附中的重要作用。突变株的细胞壁组成改变的分析使我们能够区分参与粘附的主要真菌组分，即Als蛋白和O-甘露糖基化。我们认为，测量的共粘附力参与混合生物膜的形成，因此也可能促进多微生物感染。在未来，我们预计这种SCFS平台将用于纳米医学，以破译各种病原体-病原体相互作用的分子机制，并可能有助于设计新型抗粘附剂。
Despite the clinical importance of bacterial-fungal interactions, their molecular details are poorly understood. A hallmark of such medically-important interspecies associations is the interaction between the two nosocomial pathogens Staphylococcus aureus and Candida albicans, which can lead to mixed biofilm-associated infections with enhanced antibiotic resistance. Here, we use single-cell force spectroscopy to quantify the forces engaged in bacterial-fungal co-adhesion, focusing on the poorly investigated S. epidermidis-C. albicans interaction. Force curves recorded between single bacterial and fungal germ tubes showed large adhesion forces (~5 nN) with extended rupture lengths (up to 500 nm). By contrast, bacteria poorly adhered to yeast cells, emphasizing the important role of the yeast-to-hyphae transition in mediating adhesion to bacterial cells. Analysis of mutant strains altered in cell wall composition allowed us to distinguish the main fungal components involved in adhesion, i.e. Als proteins and O-mannosylations. We suggest that the measured co-adhesion forces are involved in the formation of mixed biofilms, thus possibly as well in promoting polymicrobial infections. In the future, we anticipate that this SCFS platform will be used in nanomedicine to decipher the molecular mechanisms of a wide variety of pathogen-pathogen interactions and may help designing novel anti-adhesion agents.
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影响因子：
3.1
作者：
CARLSON, E
通讯作者：
CARLSON, E
影响因子：
17.1
作者：
Alsteens, David;Dupres, Vincent;Klotz, Stephen A.;Gaur, Nand K.;Lipke, Peter N.;Dufrene, Yves F.
通讯作者：
Dufrene, Yves F.
影响因子：
3
作者：
Kucharikova, Sona;Tournu, Helene;Bujdakova, Helena
通讯作者：
Bujdakova, Helena
影响因子：
15.9
作者：
Herriott, Melphine M.;Noverr, Mairi C.
通讯作者：
Noverr, Mairi C.
影响因子：
4.9
作者：
Harriott, Melphine M.;Noverr, Mairi C.
通讯作者：
Noverr, Mairi C.