Preventive electroacupuncture ameliorates D-galactose-induced Alzheimer's disease-like inflammation and memory deficits, probably via modulating the microbiota-gut-brain axis.
Preventive electroacupuncture ameliorates D-galactose-induced Alzheimer's disease-like inflammation and memory deficits, probably via modulating the microbiota-gut-brain axis.
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预防性电针可能通过调节微生物群-肠-脑轴改善D-半乳糖诱导的阿尔茨海默病样炎症和记忆缺陷。
DOI：
10.22038/ijbms.2021.49147.11256
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发表时间：
2021-03
影响因子：
2.2
通讯作者：
Kong LH
中科院分区：
文献类型：
--
作者：
He C;Huang ZS;Yu CC;Wang XS;Jiang T;Wu M;Kong LH
目的：观察预防性电针对D-半乳糖（D-gal）致衰老大鼠微生物群-肠-脑轴和空间学习记忆障碍的影响，并探讨其可能的作用机制。 腹腔注射D-半乳糖建立衰老模型。采用Morris水迷宫检测大鼠的空间学习记忆功能。采用RT-PCR方法检测目标肠道微生物。Western blotting检测闭锁小带1（ZO-1）和Toll样受体4（TLR 4）/核因子（NF）-κB通路蛋白的表达。采用酶联免疫吸附试验（ELISA）检测脂多糖（LPS）、二胺氧化酶（DAO）和S-100β的含量。此外，我们观察了电离钙结合适配器分子-1（Iba-1）的表达在海马CA 1区的免疫荧光。Morris水迷宫实验显示电针治疗后大鼠平均逃避潜伏期缩短，目标象限时间延长。在EA处理的大鼠中，肠道微生物群组成已经改变。分子生物学检测表明电针可提高ZO-1的表达，降低血和海马内脂多糖的含量。电针可抑制小胶质细胞的活化和TLR 4/NF-κB通路的激活。 预防性电针可减轻微生物群-肠-脑轴和空间学习记忆功能的损害，其机制可能与抑制TLR 4/NF-kB信号通路有关。穴位GV 20与ST 36合用可增强对衰老大鼠的治疗作用。
We aimed to observe the effects of preventive electroacupuncture (EA) on the microbiota–gut–brain axis and spatial learning and memory deficits and to investigate the possible mechanism using D-galactose (D-gal)-induced aging rats. D-gal was intraperitoneally injected to establish the aging model. We used Morris water maze to detect spatial learning and memory function of rats. RT-PCR was applied to test targeted gut microbes. The expression of zonula occludens-1 (ZO-1) and Toll-like receptor 4 (TLR4)/nuclear factor (NF)-κB pathway proteins were detected by Western blotting. ELISA was employed to evaluate the level of lipopolysaccharides (LPS), diamine oxidase (DAO) and S-100β. Additionally, we observed ionized calcium-binding adapter molecule-1 (Iba-1) expression in the hippocampal CA1 area by immunofluorescence. Morris water maze test showed decreased mean escape latency and increased target quadrant time after EA treatment. The gut microbiota composition has been modified in EA treated rats. Molecular examination indicated that expression of ZO-1 was improved and the the concentration of LPS in blood and hippocampus were reduced in EA treated rats. Further, we observed an inhibition of activated microglia and TLR4/NF-κB pathway in EA groups. Preventive EA may alleviate the impairments of the microbiota–gut–brain axis and spatial learning and memory in aging, and the mechanism may be related to the inhibition of TLR4/NF-kB signaling pathway. The combination of acupoints GV20 and ST36 can enhance the therapeutic effect in aging rats.
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影响因子：
3.7
作者：
Biagi E;Nylund L;Candela M;Ostan R;Bucci L;Pini E;Nikkïla J;Monti D;Satokari R;Franceschi C;Brigidi P;De Vos W
通讯作者：
De Vos W
影响因子：
2.4
作者：
Bischoff SC;Barbara G;Buurman W;Ockhuizen T;Schulzke JD;Serino M;Tilg H;Watson A;Wells JM
通讯作者：
Wells JM
影响因子：
8.1
作者：
Cani PD;Knauf C
通讯作者：
Knauf C
影响因子：
4.8
作者：
Akbari E;Asemi Z;Daneshvar Kakhaki R;Bahmani F;Kouchaki E;Tamtaji OR;Hamidi GA;Salami M
通讯作者：
Salami M
影响因子：
5.2
作者：
Cunningham KE;Turner JR
通讯作者：
Turner JR