Low limit of detection of the AlGaN/GaN-based sensor by the Kelvin connection detection technique.
Low limit of detection of the AlGaN/GaN-based sensor by the Kelvin connection detection technique.
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采用开尔文连接检测技术的 AlGaN/GaN 基传感器的检测下限
DOI：
10.1038/s41378-021-00278-7
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发表时间：
2021
影响因子：
7.9
通讯作者：
Wang P
中科院分区：
文献类型：
--
作者：
Zhang H;Gan Y;Yang S;Sheng K;Wang P
AlGaN/GaN基传感器是一种具有小型化、低成本、高灵敏度等优点的POCT（point-of-care-testing）器件。BNP是早期诊断心力衰竭的有效蛋白质生物标志物。本文提出了一种新型的具有Kelvin连接结构的AlGaN/GaN器件及其检测技术。该技术能有效地抑制背景噪声，提高信噪比。通过BNP检测实验验证了该技术的有效性。结果表明，与传统检测方法相比，该方法的检出限（LOD）由0.47 ng/mL提高到1.29 pg/mL。BNP检测实验也用具有相同表面功能化步骤的传统电化学Au电极传感器进行。AlGaN/GaN传感器显示出比Au电极传感器更好的LOD。此外，还研究了AlGaN/GaN传感器封装对背景噪声的影响，揭示了噪声源的产生机理。最后，在优化封装的基础上，确定了AlGaN/GaN传感器的最佳信噪比静态工作点。通过将传感器偏置在最佳静态工作点并将具有抗BNP的磁珠固定在AlGaN/GaN传感器的栅极上，BNP检测的LOD进一步提高到0.097 pg/mL。
The AlGaN/GaN-based sensor is a promising POCT (point-of-care-testing) device featuring miniaturization, low cost, and high sensitivity. BNP is an effective protein biomarker for the early diagnosis of HF (heart failure). In this work, a novel AlGaN/GaN device with the Kelvin connection structure and the corresponding detection technique was proposed. This technique can effectively suppress the background noise and improve the SNR (signal-to-noise ratio). A BNP detection experiment was carried out to verify the effectiveness of this technique. It is shown that compared with that of the traditional detection method, the LOD (limit of detection) was improved from 0.47 ng/mL to 1.29 pg/mL. The BNP detection experiment was also carried out with a traditional electrochemical Au-electrode sensor with the same surface functionalization steps. The AlGaN/GaN sensor showed a better LOD than the Au-electrode sensor. Moreover, the influence of AlGaN/GaN sensor package on background noise was investigated with the mechanism of the noise source revealed. Finally, based on the optimized package, the optimal SNR quiescent operating point of the AlGaN/GaN sensor was determined. By biasing the sensor at the optimal quiescent operating point and immobilizing the magnetic beads with anti-BNP on the gate of the AlGaN/GaN sensor, the LOD for BNP detection was further improved to 0.097 pg/mL.
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影响因子：
8.4
作者：
Bergveld, P
通讯作者：
Bergveld, P
影响因子：
4.6
作者：
Chen Y;Ren R;Pu H;Guo X;Chang J;Zhou G;Mao S;Kron M;Chen J
通讯作者：
Chen J
影响因子：
8.4
作者：
CHOVELON, JM;JAFFREZICRENAULT, N;PEDONE, D
通讯作者：
PEDONE, D
影响因子：
1.4
作者：
Kokawa, Takuya;Sato, Taketomo;Hashizume, Tamotsu
通讯作者：
Hashizume, Tamotsu
影响因子：
4.3
作者：
Lee, Ching-Ting;Chiu, Ying-Shuo
通讯作者：
Chiu, Ying-Shuo