A bilateral cervical contusion injury model in mice: assessment of gripping strength as a measure of forelimb motor function.
A bilateral cervical contusion injury model in mice: assessment of gripping strength as a measure of forelimb motor function.
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DOI：
10.1016/j.expneurol.2009.09.028
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发表时间：
2010-01
影响因子：
5.3
通讯作者：
Steward, Oswald
中科院分区：
文献类型：
--
作者：
Aguilar, Roberto M.;Steward, Oswald
关键词：
在这里，我们描述了一种小鼠双侧颈部挫伤模型。成年雌性小鼠使用市售撞击器（IH装置）在颈椎5级（C5）接受分级双侧挫伤。进行了三个独立的实验，以确定产生前肢功能障碍而不对一般健康造成不可接受的损害的条件。使用握力计（GSM）评估抓握能力作为前肢运动功能的量度;通过H&E和GFAP染色横截面在组织学上评估病变大小。在实验1中，小鼠受到30千达因（kdyn）的损伤;这些损伤对握力产生了最小的缺陷。在实验2中，小鼠受到75 kdyn和100 kdyn的损伤。75 kdyn的损伤在抓握中产生短暂的缺陷，在3-15 dpi之间恢复到约90%的控制; 100 kdyn的损伤产生缺陷，恢复到约50%的控制。在实验3中，没有一只受到100 kdyn损伤的小鼠恢复抓握能力。组织学评估显示分级损伤，范围从主要限于背柱（DC）的损伤到DC、灰质、腹柱和侧柱的损伤。大多数病变填充纤维组织基质，但充满液体的囊腔被发现在13%的100 kdyn损伤组和纤维填充/充满液体的囊腔的组合被发现在22%和38%的75 kdyn和100 kdyn损伤组，分别。颈部挫伤后有少量尿潴留，表明膀胱功能保留。我们的研究结果定义了产生分级的小鼠双侧颈部挫伤的条件，并证明了GSM用于评估颈部挫伤后前肢运动功能的有用性。
Here, we describe a bilateral cervical contusion model for mice. Adult female mice received graded bilateral contusion injuries at cervical level 5 (C5) using a commercially available impactor (the IH device). Three separate experiments were carried out to define conditions that produce impairments in forelimb function without unacceptable impairment of general health. A grip strength meter (GSM) was used to assess gripping ability as a measure of forelimb motor function; lesion size was assessed histologically by staining cross sections for H&E and GFAP. In Experiment 1, mice received injuries of 30 kilodynes (kdyn); these produced minimal deficits on grip strength. In Experiment 2, mice received injuries of 75 kdyn and 100 kdyn. Injuries of 75 kdyn produced transient deficits in gripping that recovered between 3-15 dpi to about 90% of control; injuries of 100 kdyn produced deficits that recovered to about 50% of control. In Experiment 3, none of the mice that received injuries of 100 kdyn recovered gripping ability. Histological assessment revealed graded injuries that ranged from damage limited primarily to the dorsal column (DC) to damage to the DC, grey matter, ventral column and lateral column. Most lesions filled in with a fibrous tissue matrix, but fluid-filled cystic cavities were found in 13% of the 100 kdyn injury group and a combination of fibrous-filled/fluid-filled cystic cavities were found in 22% and 38% of the 75 kdyn and 100 kdyn injury groups, respectively. There was minimal urine retention following cervical contusion injuries indicating preservation of bladder function. Our results define conditions to produce graded bilateral cervical contusion injuries in mice and demonstrate the usefulness of the GSM for assessing forelimb motor function after cervical contusions.
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