In vitro physicochemical characterization of five root canal sealers and their influence on an ex vivo oral multi-species biofilm community.
In vitro physicochemical characterization of five root canal sealers and their influence on an ex vivo oral multi-species biofilm community.
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五种根管封闭剂的体外理化特性及其对体外口腔多物种生物膜群落的影响。
DOI：
10.1111/iej.13742
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发表时间：
2022-07
影响因子：
5
通讯作者：
Lima, Bruno P.
中科院分区：
文献类型：
--
作者：
Saavedra, Flavia M.;Pelepenko, Lauter E.;Boyle, William S.;Zhang, Anqi;Staley, Christopher;Herzberg, Mark C.;Marciano, Marina A.;Lima, Bruno P.
目的评价5种根管封闭剂的理化性质，并评价其对无牙菌斑源性多微生物群落的影响。方法将牙菌斑来源的微生物群落暴露于密封剂（AH Plus [AHP]、GuttaFlow Bioseal [GFB]、Endoseal MTA [ESM]、Bio - C密封剂[BCS]和BioRoot RCS [BRR]）中3、6和18小时，分别采用结晶紫（CV）染色和MTT法定量密封剂对生物膜生物量和代谢活性的影响。采用变性梯度凝胶电泳（DGGE）、16S rRNA测序和扫描电镜检测生物膜群落组成和形态。遵循ISO6876:2012规范确定凝结时间，不透明度，流动性和溶解度。采用封闭的丙烯酸牙齿来评估密封剂对ph的影响。表面化学表征采用SEM和耦合能量色散光谱进行。使用Shapiro-Wilk检验评估数据的正态性。采用单维anova和Tukey试验分析凝结时间、放射不透明度、流动性和溶解度等数据。CV、MTT和ph的数据分析采用双wayanova和Dunnett检验。对16S rRNA测序数据进行α （Shannon指数和Chao分析）和β多样性（Bray-Curtis差异）分析。相似度分析评价群落组成差异（p< 0.05）。结果封口剂对微生物群落组成和形态有显著影响。所有封口机都符合ISO6876:2012对凝固时间、不透明度和流动性的要求。虽然只有AHP能有效降低生物膜生物量，但除BRR外，所有封盖剂都能降低生物膜代谢活性。结论尽管有足够的物理性能，但没有一种封口剂能阻止生物膜的生长。群落组成发生了显著变化。如果在体内观察到，这些变化可能影响管内微生物的生存、致病性和治疗结果。
AimTo evaluate the physicochemical properties of five root canal sealers and assess their effect on anex vivodental plaque‐derived polymicrobial community.MethodologyDental plaque‐derived microbial communities were exposed to the sealers (AH Plus [AHP], GuttaFlow Bioseal [GFB], Endoseal MTA [ESM], Bio‐C sealer [BCS] and BioRoot RCS [BRR]) for 3, 6 and 18 h. The sealers' effect on the biofilm biomass and metabolic activity was quantified using crystal violet (CV) staining and MTT assay, respectively. Biofilm community composition and morphology were assessed by denaturing gradient gel electrophoresis (DGGE), 16S rRNA sequencing and scanning electron microscopy. The ISO6876:2012 specifications were followed to determine the setting time, radiopacity, flowability and solubility. Obturated acrylic teeth were used to assess the sealers' effect on pH. Surface chemical characterization was performed using SEM with coupled energy‐dispersive spectroscopy. Data normality was assessed using the Shapiro–Wilk test. One‐wayanovaand Tukey's tests were used to analyze data from setting time, radiopacity, flowability and solubility. Two‐wayanovaand Dunnett's tests were used for the data analysis from CV, MTT and pH. 16S rRNA sequencing data were analyzed for alpha (Shannon index and Chao analysis) and beta diversity (Bray–Curtis dissimilarities). Differences in community composition were evaluated by analysis of similarity (p< .05).ResultsThe sealers significantly influenced microbial community composition and morphology. All sealers complied with ISO6876:2012 requirements for setting time, radiopacity and flowability. Although only AHP effectively reduced the biofilm biomass, all sealers, except BRR, reduced biofilm metabolic activity.ConclusionDespite adequate physical properties, none of the sealers tested prevented biofilm growth. Significant changes in community composition were observed. If observedin vivo, these changes could affect intracanal microbial survival, pathogenicity and treatment outcomes.
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影响因子：
9.7
作者：
Li, Y.;Carrera, C.;Chen, R.;Li, J.;Lenton, P.;Rudney, J. D.;Jones, R. S.;Aparicio, C.;Fok, A.
通讯作者：
Fok, A.
影响因子：
15.5
作者：
Edlund A;Yang Y;Hall AP;Guo L;Lux R;He X;Nelson KE;Nealson KH;Yooseph S;Shi W;McLean JS
通讯作者：
McLean JS
影响因子：
14.9
作者：
Pruesse E;Quast C;Knittel K;Fuchs BM;Ludwig W;Peplies J;Glöckner FO
通讯作者：
Glöckner FO
影响因子：
4.2
作者：
Lima AR;Ganguly T;Walker AR;Acosta N;Francisco PA;Pileggi R;Lemos JA;Gomes BPFA;Abranches J
通讯作者：
Abranches J
影响因子：
14
作者：
de Avila ED;Lima BP;Sekiya T;Torii Y;Ogawa T;Shi W;Lux R
通讯作者：
Lux R