The Cell Activation Phenomena in the Cold Atmospheric Plasma Cancer Treatment.
The Cell Activation Phenomena in the Cold Atmospheric Plasma Cancer Treatment.
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DOI：
10.1038/s41598-018-33914-w
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发表时间：
2018-10-18
影响因子：
4.6
通讯作者：
Keidar M
中科院分区：
文献类型：
--
作者：
Yan D;Xu W;Yao X;Lin L;Sherman JH;Keidar M
冷大气等离子体（CAP）是一种接近室温的电离气体。CAP是一种可控的活性物质、中性粒子、电磁场和紫外线辐射源。通过体外和体内实验证明，CAP在肿瘤治疗中具有广阔的应用前景。在这项研究中，我们首先证明了在CAP处理的癌细胞上存在活化状态，这大大降低了细胞对CAP来源的活性物质（如H2 O2和NO2−）的细胞毒性的脆弱性阈值。CAP处理的细胞毒性仍然取决于CAP来源的反应物质。癌细胞的活化状态不会引起明显的细胞毒性。这种激活是一个瞬时过程，甚至在CAP治疗开始后仅2秒就开始。在CAP治疗期间，癌细胞上的显著活化开始于10-20秒。相比之下，CAP处理后活化的脱敏需要5小时。基于CAP的细胞活化解释了直接CAP治疗对癌细胞产生比间接CAP治疗更强的细胞毒性的机制，这是理解CAP对癌细胞作用的关键。
Cold Atmospheric Plasma (CAP) is an ionized gas with a near room temperature. CAP is a controllable source for reactive species, neutral particles, electromagnetic field and UV radiation. CAP showed the promising application in cancer treatment through the demonstration in vitro and in vivo. In this study, we first demonstrate the existence of an activation state on the CAP-treated cancer cells, which drastically decreases the threshold of cell vulnerability to the cytotoxicity of the CAP-originated reactive species such as H2O2 and NO2−. The cytotoxicity of CAP treatment is still dependent on the CAP-originated reactive species. The activation state of cancer cells will not cause noticeable cytotoxicity. This activation is an instantaneous process, started even just 2 s after the CAP treatment begins. The noticeable activation on the cancer cells starts 10–20 s during the CAP treatment. In contrast, the de-sensitization of activation takes 5 hours after the CAP treatment. The CAP-based cell activation explains the mechanism by which direct CAP treatment causes a much stronger cytotoxicity over the cancer cells compared with an indirect CAP treatment do, which is a key to understand what the effect of CAP on cancer cells.
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影响因子：
3.2
作者：
Laroussi, M.;Lu, X.;Keidar, M.
通讯作者：
Keidar, M.
影响因子：
4.6
作者：
Yan D;Talbot A;Nourmohammadi N;Cheng X;Canady J;Sherman J;Keidar M
通讯作者：
Keidar M
影响因子：
6.4
作者：
Vandamme, Marc;Robert, Eric;Le Pape, Alain
通讯作者：
Le Pape, Alain
影响因子：
--
作者：
Yan D;Sherman JH;Keidar M
通讯作者：
Keidar M
影响因子：
2.2
作者：
Keidar, Michael
通讯作者：
Keidar, Michael