Calcium Dynamics of the Ventrolateral Preoptic GABAergic Neurons during Spontaneous Sleep-Waking and in Response to Homeostatic Sleep Demands.
Calcium Dynamics of the Ventrolateral Preoptic GABAergic Neurons during Spontaneous Sleep-Waking and in Response to Homeostatic Sleep Demands.
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DOI：
10.3390/ijms24098311
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发表时间：
2023-05-05
影响因子：
5.6
通讯作者：
Alam, Md. Noor
中科院分区：
文献类型：
--
作者：
Kostin, Andrey;Alam, Md. Aftab;Saevskiy, Anton;Yang, Chenyi;Golshani, Peyman;Alam, Md. Noor
腹外侧视前区（VLPO）含有GABA能睡眠激活神经元。然而，这些神经元参与表达自发睡眠和稳态睡眠调节需求的程度还没有完全了解。我们使用钙（Ca 2+）成像来表征VLPO神经元的活动动力学，特别是那些表达囊泡GABA转运蛋白（VGAT）的神经元在自发性睡眠-觉醒和对稳态睡眠需求的反应。用GCaMP 6转染野生型和VGAT-Cre小鼠的VLPO，并在自发睡眠-觉醒和3 h睡眠剥夺（SD）后1 h恢复睡眠期间记录未鉴定（UNID）和VGAT细胞的Ca 2+荧光。尽管VGAT和UNID神经元在睡眠-觉醒期间均表现出异质性Ca 2+荧光，但大多数VLPO神经元在非REM/REM（VGAT，120/303; UNID，39/106）和REM睡眠（VGAT，32/303; UNID，19/106）期间表现出增加的活性。与基线清醒相比，VLPO睡眠活性神经元（n = 91）表现出更高的活性，随着SD的增加，在恢复期间保持升高。这些神经元在非REM睡眠期间也表现出增加的Ca 2+荧光，其特征是SD后恢复期间慢波活动和REM睡眠增加。这些发现支持了这样的观点，即VLPO睡眠活性神经元，包括GABA能神经元，是神经元回路的组成部分，介导自发睡眠和对持续觉醒的稳态反应。
The ventrolateral preoptic area (VLPO) contains GABAergic sleep-active neurons. However, the extent to which these neurons are involved in expressing spontaneous sleep and homeostatic sleep regulatory demands is not fully understood. We used calcium (Ca2+) imaging to characterize the activity dynamics of VLPO neurons, especially those expressing the vesicular GABA transporter (VGAT) across spontaneous sleep-waking and in response to homeostatic sleep demands. The VLPOs of wild-type and VGAT-Cre mice were transfected with GCaMP6, and the Ca2+ fluorescence of unidentified (UNID) and VGAT cells was recorded during spontaneous sleep-waking and 3 h of sleep deprivation (SD) followed by 1 h of recovery sleep. Although both VGAT and UNID neurons exhibited heterogeneous Ca2+ fluorescence across sleep-waking, the majority of VLPO neurons displayed increased activity during nonREM/REM (VGAT, 120/303; UNID, 39/106) and REM sleep (VGAT, 32/303; UNID, 19/106). Compared to the baseline waking, VLPO sleep-active neurons (n = 91) exhibited higher activity with increasing SD that remained elevated during the recovery period. These neurons also exhibited increased Ca2+ fluorescence during nonREM sleep, marked by increased slow-wave activity and REM sleep during recovery after SD. These findings support the notion that VLPO sleep-active neurons, including GABAergic neurons, are components of neuronal circuitry that mediate spontaneous sleep and homeostatic responses to sustained wakefulness.
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影响因子：
3.5
作者：
Saito YC;Tsujino N;Hasegawa E;Akashi K;Abe M;Mieda M;Sakimura K;Sakurai T
通讯作者：
Sakurai T
DOI：
10.1152/ajpregu.2000.279.6.r2079
发表时间：
2000-12-01
影响因子：
2.8
作者：
Gong, H;Szymusiak, R;McGinty, D
通讯作者：
McGinty, D
影响因子：
14.8
作者：
Resendez SL;Jennings JH;Ung RL;Namboodiri VM;Zhou ZC;Otis JM;Nomura H;McHenry JA;Kosyk O;Stuber GD
通讯作者：
Stuber GD
影响因子：
2.9
作者：
Guzmán-Marín, R;Alam, MN;McGinty, D
通讯作者：
McGinty, D
影响因子：
3.3
作者：
Sakai, K.
通讯作者：
Sakai, K.