An Implantable System For Chronic In Vivo Electromyography.
An Implantable System For Chronic In Vivo Electromyography.
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DOI：
10.3791/60345
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发表时间：
2020-04-21
期刊：
影响因子：
--
通讯作者：
Huang S
中科院分区：
文献类型：
--
作者：
Zealear D;Li Y;Huang S
肌电图（EMG）测量肌肉对电刺激或运动单位的自发活动的反应，并在评估神经肌肉功能中起重要作用。由于传统EMG记录技术的侵入性，反映神经损伤后肌肉神经再支配状态的EMG活动的慢性记录受到限制。在这方面，植入式系统被设计用于长期的体内EMG记录和神经刺激。它已被应用和测试在喉肌神经再支配的研究。该系统包括：1）两个双极电极神经袖带和电极导线，用于刺激两条神经中的每一条：喉返神经（RLN）和喉上级神经（SLN）的内部分支; 2）两个EMG记录电极和电极导线，用于两条喉肌中的每一条：环杓后肌（PCA）和甲杓-环杓外侧肌复合体（TA-LCA）;以及3）皮肤插座，其使用连接电缆将所有植入的引线端子连接到外部记录仪和刺激器。引线为聚四氟乙烯涂层、多丝、316型不锈钢。它们是盘绕的，并且可以在清醒动物的身体运动期间拉伸，以防止导线断裂和电极移位。该系统在无菌手术期间植入。然后，在第二次手术中切断RLN之前进行基线EMG记录以研究肌肉神经再支配。在整个研究过程中，在麻醉动物中进行多个生理会话，以获得反映喉肌神经再支配状态的诱发和自发EMG活动。该系统结构紧凑，在研究过程中无感染，并且非常耐用。这种植入式系统可以为需要在麻醉或自由移动的动物中进行长期记录或神经刺激的研究提供可靠的平台。
Electromyography (EMG) measures the muscle response to electrical stimulation or spontaneous activity of motor units and plays an important role in assessing neuromuscular function. Chronic recording of EMG activity reflecting a muscle’s reinnervation status after nerve injury has been limited, due to the invasive nature of traditional EMG recording techniques. In this regard, an implantable system is designed for long-term, in vivo EMG recording and nerve stimulation. It has been applied and tested in a study on reinnervation of laryngeal muscles. This system consists of 1) two bipolar electrode nerve cuffs and leads for stimulating each of two nerves: the recurrent laryngeal nerve (RLN) and internal branch of the superior laryngeal nerve (SLN); 2) two EMG recording electrodes and leads for each of the two laryngeal muscles: posterior cricoarytenoid (PCA) muscle and thyroarytenoid-lateral cricoarytenoid (TA-LCA) muscle complex; and 3) a skin receptacle interfacing all implanted lead terminals to an external recording preamplifier and stimulator using a connection cable. The wire leads are Teflon-coated, multi-filament, type 316 stainless steel. They are coiled and can stretch during body movement of the awake animal to prevent lead breakage and electrode migration. This system is implanted during an aseptic surgery. Afterwards, baseline EMG recordings are performed before the RLN is transected in the second surgery to study muscle reinnervation. Throughout the study, multiple physiological sessions are conducted in the anesthetized animal to obtain evoked and spontaneous EMG activity that reflects the reinnervation status of laryngeal muscles. The system is compact, free of infection over the course of the study, and highly durable. This implantable system can provide a reliable platform for research in which long-term recording or nerve stimulation is required in an anesthetized or freely moving animal.
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影响因子：
2.5
作者：
Zealear, DL;Rodriguez, RJ;Garren, KC
通讯作者：
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3.4
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3.3
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影响因子：
2.4
作者：
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