Enhanced skeletal muscle glycogen repletion after endurance exercise is associated with higher plasma insulin and skeletal muscle hexokinase 2 protein levels in mice: comparison of level running and downhill running model
Enhanced skeletal muscle glycogen repletion after endurance exercise is associated with higher plasma insulin and skeletal muscle hexokinase 2 protein levels in mice: comparison of level running and downhill running model
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耐力运动后骨骼肌糖原补充增强与小鼠血浆胰岛素和骨骼肌己糖激酶 2 蛋白水平升高相关：水平跑和下坡跑模型的比较
DOI：
10.1007/s13105-021-00806-z
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发表时间：
2021
影响因子：
3.4
通讯作者：
Hideo Hatta
中科院分区：
文献类型：
--
作者：
Yumiko Takahashi;Juli Sarkar;Jumpei Yamada;Yutaka Matsunaga;Yudai Nonaka;Mai Banjo;Ryo Sakaguchi;Terunaga Shinya;Hideo Hatta
为了确定影响运动后肌糖原补充的因素，我们比较了水平跑和下坡跑后的糖原恢复，这是一种运动后糖原恢复受损的实验模型。雄性癌症研究所（ICR）小鼠在跑步机上进行耐力水平跑步（无倾斜）或下坡跑步（-5 °倾斜）。在实验1中，为了确定这两种类型的运动是否导致不同的运动后糖原补充模式，在运动后立即或运动后2天收获组织。与对照组（久坐）相比，水平跑在运动后2天诱导比目鱼肌显著的糖原超补偿（p= 0.002）。下坡跑并没有引起糖原的过度补偿。在实验2中，小鼠在运动后1天口服葡萄糖;这仅在水平跑步组中诱导比目鱼肌和跖肌中的糖原超补偿（比目鱼肌：p= 0.005，跖肌：p= 0.003）。与下坡跑相比，水平跑对血浆胰岛素（p= 0.017）和C肽浓度（p= 0.011）有显著的正主效应。在骨骼肌中，葡萄糖转运蛋白4水平或与胰岛素信号传导和代谢相关的蛋白质的磷酸化状态没有差异。水平跑步组在运动后1天比目鱼肌（p= 0.046）和跖肌（p=0.044）中的己糖激酶2（HK 2）蛋白含量显著高于下坡跑步组。我们的研究结果表明，运动后骨骼肌糖原补充可能部分地受到血浆胰岛素和骨骼肌HK 2蛋白水平的影响。
To identify factors that influence post-exercise muscle glycogen repletion, we compared the glycogen recovery after level running with downhill running, an experimental model of impaired post-exercise glycogen recovery. Male Institute of Cancer Research (ICR) mice performed endurance level running (no inclination) or downhill running (−5° inclination) on a treadmill. In Experiment 1, to determine whether these two types of exercise resulted in different post-exercise glycogen repletion patterns, tissues were harvested immediately post-exercise or 2 days post-exercise. Compared to the control (sedentary) group, level running induced significant glycogen supercompensation in the soleus muscle at 2 days post-exercise (p= 0.002). Downhill running did not induce glycogen supercompensation. In Experiment 2, mice were orally administered glucose 1 day post-exercise; this induced glycogen supercompensation in soleus and plantaris muscle only in the level running group (soleus:p= 0.005, plantaris:p= 0.003). There were significant positive main effects of level running compared to downhill running on the plasma insulin (p= 0.017) and C-peptide concentration (p= 0.011). There was no difference in the glucose transporter 4 level or the phosphorylated states of proteins related to insulin signaling and metabolism in skeletal muscle. The level running group showed significantly higher hexokinase 2 (HK2) protein content in both soleus (p= 0.046) and plantaris muscles (p=0.044) at 1 day after exercise compared to the downhill running group. Our findings suggest that post-exercise skeletal muscle glycogen repletion might be partly influenced by plasma insulin and skeletal muscle HK2 protein levels.
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在不同倾斜度上运动期间大鼠肌肉中糖原的损失。
DOI：
10.1242/jeb.176.1.135
发表时间：
1993
期刊：
The Journal of experimental biology
影响因子：
--
作者：
Armstrong,RB;Taylor,CR
通讯作者：
Taylor,CR
影响因子：
3.3
作者：
ARMSTRONG, RB;OGILVIE, RW;SCHWANE, JA
通讯作者：
SCHWANE, JA
离心运动后糖原积累的时间过程。
影响因子：
3.3
作者：
J. Widrick;D. Costill;Glenn K. McConell;D. E. Anderson;D. Pearson;J J Zachwieja
通讯作者：
J J Zachwieja
偏心肌肉损伤会暂时降低大鼠骨骼肌 GLUT-4 蛋白。
影响因子：
3.3
作者：
S. Asp;S. Kristiansen;Erik A. Richter
通讯作者：
Erik A. Richter
影响因子：
5.5
作者：
ASP, S;DAUGAARD, JR;RICHTER, EA
通讯作者：
RICHTER, EA