Maintenance and turnover of Sox2+ adult stem cells in the gustatory epithelium.
Maintenance and turnover of Sox2+ adult stem cells in the gustatory epithelium.
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DOI：
10.1371/journal.pone.0267683
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发表时间：
2022
期刊：
影响因子：
3.7
通讯作者：
中科院分区：
文献类型：
--
作者：
味蕾细胞在口腔中的持续周转是味觉组织动态平衡的基础。以往的研究表明，Sox2+干细胞在口腔上皮细胞中可以分化为各种类型的上皮细胞，包括味蕾细胞和非味觉上皮细胞，而Sox2是味蕾细胞生成所必需的。在这里，我们通过对Sox2-CreERT2；rosa26-五彩纸屑小鼠的多色谱系追踪分析，展示了单个干细胞的动态。在非味觉上皮中，随着时间的推移，由表达相同荧光蛋白的细胞簇填充的单色区逐渐生长，而通过短期示踪，上皮细胞被随机标记多个荧光蛋白。在味觉上皮细胞中也观察到类似的现象。这些结果表明，Sox2+干细胞种群是通过平衡某些干细胞的增加和其他干细胞的减少来维持的。在味觉上皮细胞中，许多单个味蕾含有标记有不同荧光蛋白的细胞，这表明单个味蕾由来自多个Sox2+干细胞的细胞组成。我们的结果揭示了Sox2+干细胞在味蕾细胞周转和味觉组织内稳的基础上的特征。
Continuous turnover of taste bud cells in the oral cavity underlies the homeostasis of taste tissues. Previous studies have demonstrated that Sox2+ stem cells give rise to all types of epithelial cells including taste bud cells and non-gustatory epithelial cells in the oral epithelium, and Sox2 is required for generating taste bud cells. Here, we show the dynamism of single stem cells through multicolor lineage tracing analyses in Sox2-CreERT2; Rosa26-Confetti mice. In the non-gustatory epithelium, unicolored areas populated by a cluster of cells expressing the same fluorescent protein grew over time, while epithelial cells were randomly labeled with multiple fluorescent proteins by short-term tracing. Similar phenomena were observed in gustatory epithelia. These results suggest that the Sox2+ stem cell population is maintained by balancing the increase of certain stem cells with the reduction of the others. In the gustatory epithelia, many single taste buds contained cells labeled with different fluorescent proteins, indicating that a single taste bud is composed of cells derived from multiple Sox2+ stem cells. Our results reveal the characteristics of Sox2+ stem cells underlying the turnover of taste bud cells and the homeostasis of taste tissues.
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