Functional alleles of the flowering time regulator FRIGIDA in the Brassica oleracea genome.
Functional alleles of the flowering time regulator FRIGIDA in the Brassica oleracea genome.
复制标题
DOI：
10.1186/1471-2229-12-21
复制
发表时间：
2012-02-14
影响因子：
5.3
通讯作者：
Dean C
中科院分区：
文献类型：
--
作者：
Irwin JA;Lister C;Soumpourou E;Zhang Y;Howell EC;Teakle G;Dean C
植物采取不同的繁殖策略，以适应在一系列气候条件下的生长。在拟南芥中，FRI通过增加MADS盒转录抑制子FLOWERLOCUS C（FLC）的表达来赋予春化要求，从而赋予冬季一年生习性。FRI变异在A.拟南芥生活史策略，作为独立的功能丧失等位基因，在不同的加入，导致一个快速循环的习惯，似乎已经进化了很多次。本研究的目的是鉴定和表征甘蓝中FRI的直向同源物。我们描述了甘蓝FRI的特性，并确定了两个B。甘蓝FRI直向同源物（BolC.FRI. a和BolC.FRI. B）。这些结果表明，FRI与其他菊科植物（包括A. thaliana，但具有分叉的N末端。这些基因定位于B的三个区域中的两个。oleracea染色体与A. thaliana 5号染色体的突变，表明FRI拷贝之一在形成B的古代三倍体事件后丢失。甘蓝基因组该基因组位置与A中的FRI不同线。thaliana和比较分析揭示了重组事件内A.拟南芥FRI启动子。这是重新定位A。thaliana FRI位于4号染色体上，非常靠近核仁组织者区，在A.拟南芥染色体5。我们的数据表明这种重排发生在从A发散之后。抒情琴。我们探索了栽培B内BolC. FRI. a的等位基因变异。oleracea种质，并确定了两个主要的等位基因，这似乎是同等功能的彼此和A。thaliana FRI融合蛋白在A. thaliana.我们鉴定了两个甘蓝FRI基因，其中一个我们通过A. thaliana的互补实验是功能性的，并表明它们的基因组位置与A. thaliana FRI是由于一个古老的重组事件。这使得以前的关联分析FRI与芸苔属的生活史策略的变化。
Plants adopt different reproductive strategies as an adaptation to growth in a range of climates. In Arabidopsis thaliana FRIGIDA (FRI) confers a vernalization requirement and thus winter annual habit by increasing the expression of the MADS box transcriptional repressor FLOWERING LOCUS C (FLC). Variation at FRI plays a major role in A. thaliana life history strategy, as independent loss-of-function alleles that result in a rapid-cycling habit in different accessions, appear to have evolved many times. The aim of this study was to identify and characterize orthologues of FRI in Brassica oleracea. We describe the characterization of FRI from Brassica oleracea and identify the two B. oleracea FRI orthologues (BolC.FRI.a and BolC.FRI.b). These show extensive amino acid conservation in the central and C-terminal regions to FRI from other Brassicaceae, including A. thaliana, but have a diverged N-terminus. The genes map to two of the three regions of B. oleracea chromosomes syntenic to part of A. thaliana chromosome 5 suggesting that one of the FRI copies has been lost since the ancient triplication event that formed the B. oleracea genome. This genomic position is not syntenic with FRI in A. thaliana and comparative analysis revealed a recombination event within the A. thaliana FRI promoter. This relocated A. thaliana FRI to chromosome 4, very close to the nucleolar organizer region, leaving a fragment of FRI in the syntenic location on A. thaliana chromosome 5. Our data show this rearrangement occurred after the divergence from A. lyrata. We explored the allelic variation at BolC.FRI.a within cultivated B. oleracea germplasm and identified two major alleles, which appear equally functional both to each other and A. thaliana FRI, when expressed as fusions in A. thaliana. We identify the two Brassica oleracea FRI genes, one of which we show through A. thaliana complementation experiments is functional, and show their genomic location is not syntenic with A. thaliana FRI due to an ancient recombination event. This has complicated previous association analyses of FRI with variation in life history strategy in the Brassica genus.
登录
查看更多内容
影响因子：
64.8
作者：
Hayama, R;Yokoi, S;Shimamoto, K
通讯作者：
Shimamoto, K
影响因子：
5.4
作者：
Gao, Muqiang;Li, Genyi;Quiros, Carlos
通讯作者：
Quiros, Carlos
影响因子：
56.9
作者：
Johanson, U;West, J;Dean, C
通讯作者：
Dean, C
影响因子：
5.4
作者：
Allender, C. J.;Allainguillaume, J.;King, G. J.
通讯作者：
King, G. J.
DOI：
10.1073/pnas.0909766107
发表时间：
2010-10-26
影响因子：
11.1
作者：
Beilstein, Mark A.;Nagalingum, Nathalie S.;Mathews, Sarah
通讯作者：
Mathews, Sarah