Spatial organization of a ubiquitous eukaryotic kinetochore protein network in Drosophila chromosomes.
Spatial organization of a ubiquitous eukaryotic kinetochore protein network in Drosophila chromosomes.
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DOI：
10.1007/s00412-007-0103-y
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发表时间：
2007-08
期刊：
影响因子：
1.6
通讯作者：
Lehner CF
中科院分区：
文献类型：
--
作者：
Schittenhelm RB;Heeger S;Althoff F;Walter A;Heidmann S;Mechtler K;Lehner CF
在减数分裂和有丝分裂过程中，染色体的分离依赖于着丝粒区域内功能性动粒的组装。尽管着丝粒DNA和动粒蛋白质具有基本的生物学作用，但它们的序列保守性令人惊讶地小。然而，我们在黑腹果蝇中鉴定到目前为止鉴定的最分歧的直系同源物，其编码包括Ndc80和Mis复合物的动粒蛋白网络的组分，进一步强调了共享真核动粒设计的概念。为了确定其空间组织，我们已经分析了数以百计的本地染色体从转基因果蝇株共表达红色和绿色荧光融合蛋白的组合，完全能够提供必要的野生型功能的定量光学显微镜。因此，Cenp-A/Cid，Cenp-C，Mis12和Ndc80复合物沿着姐妹间动粒轴以低于10 nm的分辨率作图。发现Cenp-C的C末端靠近但与最内部的组分Cenp-A/Cid很好地分离。Cenp-C的N末端更靠外，与Mis12和杆状Ndc80复合物的Spc25末端成簇，已知其在另一个更远的Ndc80/Nuf2末端与微管结合。
Chromosome segregation during meiosis and mitosis depends on the assembly of functional kinetochores within centromeric regions. Centromeric DNA and kinetochore proteins show surprisingly little sequence conservation despite their fundamental biological role. However, our identification in Drosophila melanogaster of the most diverged orthologs identified so far, which encode components of a kinetochore protein network including the Ndc80 and Mis complexes, further emphasizes the notion of a shared eukaryotic kinetochore design. To determine its spatial organization, we have analyzed by quantitative light microscopy hundreds of native chromosomes from transgenic Drosophila strains coexpressing combinations of red and green fluorescent fusion proteins, fully capable of providing the essential wild-type functions. Thereby, Cenp-A/Cid, Cenp-C, Mis12 and the Ndc80 complex were mapped along the inter sister kinetochore axis with a resolution below 10 nm. The C terminus of Cenp-C was found to be near but well separated from the innermost component Cenp-A/Cid. The N terminus of Cenp-C is further out, clustered with Mis12 and the Spc25 end of the rod-like Ndc80 complex, which is known to bind to microtubules at its other more distal Ndc80/Nuf2 end.
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影响因子：
10.5
作者：
Heeger, S;Leismann, O;Lehner, CF
通讯作者：
Lehner, CF
影响因子：
2.9
作者：
BLUM, H;BEIER, H;GROSS, HJ
通讯作者：
GROSS, HJ
影响因子：
14.9
作者：
Biegert A;Mayer C;Remmert M;Söding J;Lupas AN
通讯作者：
Lupas AN
DOI：
10.1083/jcb.200210005
发表时间：
2003-01-06
期刊：
The Journal of cell biology
影响因子：
--
作者：
Goshima G;Kiyomitsu T;Yoda K;Yanagida M
通讯作者：
Yanagida M
影响因子：
4.8
作者：
Ciferri, C;De Luca, J;Musacchio, A
通讯作者：
Musacchio, A