Utilization of microparticles in next-generation assays for microflow cytometers.

Utilization of microparticles in next-generation assays for microflow cytometers.
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DOI:
10.1007/s00216-010-3848-8
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发表时间:
2010-11
影响因子:
4.3
通讯作者:
Ligler, Frances S.
Ligler, Frances S.
中科院分区:
化学2区
文献类型:
--
作者:
Kim, Jason S.;Ligler, Frances S.

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Micron-sized particles have primarily been used in microfabricated flow cytometers for calibration purposes and proof-of-concept experiments. With increasing frequency, microparticles are serving as a platform for assays measured in these small analytical devices. Light scattering has been used to measure the agglomeration of antibody-coated particles in the presence of an antigen. Impedance detection is another technology being integrated into microflow cytometers for microparticle-based assays. Fluorescence is the most popular detection method in flow cytometry, enabling highly sensitive multiplexed assays. Finally, magnetic particles have also been used to measure antigen levels using a magnetophoretic micro-device. We review the progress of microparticle-based assays in micro-flow cytometry in terms of the advantages and limitations of each approach.
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