Lactobacillus crispatus dominant vaginal microbiome is associated with inhibitory activity of female genital tract secretions against Escherichia coli.
Lactobacillus crispatus dominant vaginal microbiome is associated with inhibitory activity of female genital tract secretions against Escherichia coli.
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DOI：
10.1371/journal.pone.0096659
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发表时间：
2014
期刊：
影响因子：
3.7
通讯作者：
Burk RD
中科院分区：
文献类型：
--
作者：
Ghartey JP;Smith BC;Chen Z;Buckley N;Lo Y;Ratner AJ;Herold BC;Burk RD
女性生殖道分泌物抑制E。该活性可以防止定植并提供健康微生物组的生物标志物。我们假设高E。大肠杆菌的抑制活性将与卷曲乳杆菌和/或詹氏菌占优势的微生物组相关，并且不同于具有低抑制活性的女性。根据具有高（>90%抑制）或低（<20%抑制）抗E选择来自先前在近足月妊娠和非妊娠健康女性的横断面研究中获得的20个样品的阴道拭子细胞团块。coli活性。使用Illumina HiSeq 2000平台扩增16 S核糖体RNA基因的V6区并测序。还测定了来自卷曲乳杆菌、惰性乳杆菌和阴道加德纳菌的过滤培养物上清液中的E. coli抑制活性。16份样品（10份具有高活性，6份具有低活性）产生了可评价的微生物组数据。与非妊娠女性相比，妊娠女性的主要微生物组物种没有差异（各n=8）。 然而，与低E相比，高E的女性之间存在显着差异。coli抑制活性。高活性与L的优势有关。卷曲螺旋体（p<0.007）和L. crispatus的E.大肠杆菌的抑制活性相比，从L. iners或G.流浪汉值得注意的是，E.不同菌株对大肠杆菌的抑制活性不同。卷曲的 微生物群落丰富的L。crispatus可能是E.大肠杆菌抑制活性的阴道分泌物和努力，以促进这种环境可能会防止大肠杆菌。大肠杆菌定植和相关后遗症，包括早产。
Female genital tract secretions inhibit E. coli ex vivo and the activity may prevent colonization and provide a biomarker of a healthy microbiome. We hypothesized that high E. coli inhibitory activity would be associated with a Lactobacillus crispatus and/or jensenii dominant microbiome and differ from that of women with low inhibitory activity. Vaginal swab cell pellets from 20 samples previously obtained in a cross-sectional study of near-term pregnant and non-pregnant healthy women were selected based on having high (>90% inhibition) or low (<20% inhibition) anti-E. coli activity. The V6 region of the 16S ribosomal RNA gene was amplified and sequenced using the Illumina HiSeq 2000 platform. Filtered culture supernatants from Lactobacillus crispatus, Lactobacillus iners, and Gardnerella vaginalis were also assayed for E. coli inhibitory activity. Sixteen samples (10 with high and 6 with low activity) yielded evaluable microbiome data. There was no difference in the predominant microbiome species in pregnant compared to non-pregnant women (n = 8 each). However, there were significant differences between women with high compared to low E. coli inhibitory activity. High activity was associated with a predominance of L. crispatus (p<0.007) and culture supernatants from L. crispatus exhibited greater E. coli inhibitory activity compared to supernatants obtained from L. iners or G. vaginalis. Notably, the E. coli inhibitory activity varied among different strains of L. crispatus. Microbiome communities with abundant L. crispatus likely contribute to the E. coli inhibitory activity of vaginal secretions and efforts to promote this environment may prevent E. coli colonization and related sequelae including preterm birth.
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细菌性阴道病女性的细菌群落：高分辨率系统发育分析揭示了微生物群与临床标准的关系。
影响因子：
3.7
作者：
Srinivasan S;Hoffman NG;Morgan MT;Matsen FA;Fiedler TL;Hall RW;Ross FJ;McCoy CO;Bumgarner R;Marrazzo JM;Fredricks DN
通讯作者：
Fredricks DN
影响因子：
10.5
作者：
Ma B;Forney LJ;Ravel J
通讯作者：
Ravel J
DOI：
10.1126/science.1224203
发表时间：
2012-06-08
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Costello EK;Stagaman K;Dethlefsen L;Bohannan BJ;Relman DA
通讯作者：
Relman DA
影响因子：
3.7
作者：
Kalyoussef S;Nieves E;Dinerman E;Carpenter C;Shankar V;Oh J;Burd B;Angeletti RH;Buckheit KW;Fredricks DN;Madan RP;Keller MJ;Herold BC
通讯作者：
Herold BC
影响因子：
3.7
作者：
Aagaard K;Riehle K;Ma J;Segata N;Mistretta TA;Coarfa C;Raza S;Rosenbaum S;Van den Veyver I;Milosavljevic A;Gevers D;Huttenhower C;Petrosino J;Versalovic J
通讯作者：
Versalovic J