Frizzled 2 Functions in the Regulation of TOR-Mediated Embryonic Development and Fecundity in Cyrtorhinus lividipennis Reuter.
Frizzled 2 Functions in the Regulation of TOR-Mediated Embryonic Development and Fecundity in Cyrtorhinus lividipennis Reuter.
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Frizzled 2 在 TOR 介导的 Cyrtorhinus lividipennis Reuter 胚胎发育和繁殖力调节中的功能。
DOI：
10.3389/fphys.2020.579233
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发表时间：
2020
影响因子：
4
通讯作者：
Liu F
中科院分区：
文献类型：
--
作者：
Ge L;Jiang L;Zheng S;Zhou Y;Wu Q;Liu F
这种名为Cyrtorhinus lividipennis Reuter的盲蝽是亚洲水稻飞虱的重要捕食者。在之前的一项研究中，与以水稻为食的褐飞虱（BPH）卵相比，以禾草类杂草为食的C. lividipennis发育迟缓。在本研究中，水稻中选择的氨基酸（AAs）浓度高于5种禾本科植物，这可能解释了C. lividipennis在水稻上的生长促进。饲喂aa缺失人工饲料时，Wnt/β-catenin通路受到抑制。此外，沉默frzzled2 （Fz2）表达的雌C. lividipennis显示Wnt/β-catenin和雷帕霉素（rapamycin， TOR）通路靶点显著降低。沉默Fz2导致卵黄蛋白原基因（Vg）表达降低，卵黄蛋白原基因在卵母细胞中的积累减少，卵巢和脂肪体中可溶性蛋白减少，幼崽激素滴度降低，产卵前期延长，捕食能力、体重和卵数降低。Fz2沉默导致卵巢发育不全，卵巢卵母细胞生长受到抑制，导致子代数量减少，孵化率降低。Fz2基因的沉默也会导致眼斑和器官发育不全的畸形胚胎，这表明Fz2基因是胚胎发育、卵发生和卵子成熟的重要基因。综上所述，本研究建立了Wnt和TOR通路之间的潜在联系，它们在响应AA信号时协同作用调节C. lividipennis繁殖。这些研究结果为大规模养殖褐飞虱天敌提供了有价值的新信息，对减少稻田飞虱危害具有重要意义。
The mirid bug, Cyrtorhinus lividipennis Reuter, is an important predator of rice planthoppers in Asia. In a previous study, C. lividipennis fed on gramineous weeds with brown planthopper (BPH) eggs had reduced development compared to those fed on rice with BPH eggs. In the current study, the concentrations of selected amino acids (AAs) were higher in rice than five gramineous species, which might explain the enhanced growth of C. lividipennis on rice. When C. lividipennis was fed on AA-deprived artificial diets, the Wnt/β-catenin pathway was inhibited. Furthermore, C. lividipennis females silenced for expression of Frizzled 2 (Fz2) showed a significant reduction in the Wnt/β-catenin and target of rapamycin (TOR) pathways. Silencing Fz2 led to decreased expression of the vitellogenin gene (Vg), lower Vg accumulation in oocytes, reduced soluble protein in ovaries and fat bodies, reduced titers of juvenile hormone, prolonged preoviposition periods, and lower predation capacity, body weight, and egg numbers as controlled to controls. Fz2 silencing resulted in undeveloped ovaries and the inhibition of oocyte growth in the ovarioles, resulting in decreased numbers of offspring and reduced hatching rates. The silencing of Fz2 also resulted in aberrant embryos with undeveloped eyespots and organs, suggesting that Fz2 is an essential gene for embryonic development, oogenesis, and egg maturation. In summary, this study established a potential link between Wnt and TOR pathways, which interact synergistically to regulate C. lividipennis reproduction in response to AA signals. These results provide valuable new information that can be applied to large-scale rearing of C. lividipennis predators, which is important for reducing planthopper damage in rice fields.
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影响因子：
3.7
作者：
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通讯作者：
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影响因子：
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DOI：
10.1073/pnas.2235649100
发表时间：
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影响因子：
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作者：
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影响因子：
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作者：
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作者：
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