Heterogeneous glycation of cancellous bone and its association with bone quality and fragility.
Heterogeneous glycation of cancellous bone and its association with bone quality and fragility.
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DOI：
10.1371/journal.pone.0035047
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发表时间：
2012
期刊：
影响因子：
3.7
通讯作者：
Vashishth D
中科院分区：
文献类型：
--
作者：
Karim L;Vashishth D
非酶糖化 (NEG) 和酶生化过程会产生交联，从而改变细胞外基质 (ECM) 并影响骨组织的周转。由于 NEG 影响周转，并且局部水平的周转影响微结构以及微损伤的形成和去除，因此我们假设松质骨中的 NEG 是异质的，并且部分解释了微结构和微损伤对骨脆性的影响。来自 23 名捐赠者的人类小梁骨芯接受了压缩测试。经过机械测试的岩心以及另外 19 个岩心用醋酸双氧铀染色并成像以确定微结构并测量微损伤。测量屈服后的机械性能，并从样本子集中提取受损的小梁，并表征诱导微损伤的形态。使用比色测定和超高效液相色谱 (UPLC) 对测试样本和提取的小梁的酶促和非酶促交联含量进行定量。结果表明，酶交联的增加对骨骼有益，与骨骼韧性的增加和微损伤的减少有关。相反，NEG 增加的骨骼达到破坏所需的应变较小，并且韧性较差。 NEG 异质改性小梁微结构，其中在小梁棒中发现大量 NEG 交联，并具有机械有害形式的微损伤（线性微裂纹）。胫骨松质骨中 NEG 的程度是骨脆性的主要预测因子，并且与微结构和微损伤的变化相关。
Non-enzymatic glycation (NEG) and enzymatic biochemical processes create crosslinks that modify the extracellular matrix (ECM) and affect the turnover of bone tissue. Because NEG affects turnover and turnover at the local level affects microarchitecture and formation and removal of microdamage, we hypothesized that NEG in cancellous bone is heterogeneous and accounts partly for the contribution of microarchitecture and microdamage on bone fragility. Human trabecular bone cores from 23 donors were subjected to compression tests. Mechanically tested cores as well as an additional 19 cores were stained with lead-uranyl acetate and imaged to determine microarchitecture and measure microdamage. Post-yield mechanical properties were measured and damaged trabeculae were extracted from a subset of specimens and characterized for the morphology of induced microdamage. Tested specimens and extracted trabeculae were quantified for enzymatic and non-enzymatic crosslink content using a colorimetric assay and Ultra-high Performance Liquid Chromatography (UPLC). Results show that an increase in enzymatic crosslinks was beneficial for bone where they were associated with increased toughness and decreased microdamage. Conversely, bone with increased NEG required less strain to reach failure and were less tough. NEG heterogeneously modified trabecular microarchitecture where high amounts of NEG crosslinks were found in trabecular rods and with the mechanically deleterious form of microdamage (linear microcracks). The extent of NEG in tibial cancellous bone was the dominant predictor of bone fragility and was associated with changes in microarchitecture and microdamage.
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影响因子：
4.4
作者：
Rath, NC;Balog, JM;Tierce, JF
通讯作者：
Tierce, JF
影响因子：
15.9
作者：
HUI, SL;SLEMENDA, CW;JOHNSTON, CC
通讯作者：
JOHNSTON, CC
DOI：
10.1302/0301-620x.79b1.6978
发表时间：
1997-01-01
影响因子：
--
作者：
Kuster, MS;Wood, GA;Gachter, A
通讯作者：
Gachter, A
影响因子：
4.1
作者：
KNOTT, L;WHITEHEAD, CC;BAILEY, AJ
通讯作者：
BAILEY, AJ
影响因子：
2.8
作者：
Karim, Lamya;Vashishth, Deepak
通讯作者：
Vashishth, Deepak