Knee loading stimulates cortical bone formation in murine femurs.
Knee loading stimulates cortical bone formation in murine femurs.
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DOI：
10.1186/1471-2474-7-73
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发表时间：
2006-09-19
影响因子：
2.3
通讯作者：
Yokota H
中科院分区：
文献类型：
--
作者：
Zhang P;Su M;Tanaka SM;Yokota H
骨改变其结构和质量，以响应机械环境，因此不同的加载方式已被检查，研究负载驱动的骨形成。当前研究旨在评估膝关节负荷对股骨骨干皮质骨的合成代谢影响。使用定制的压电加载器，以5、10、15和20 Hz的频率向C57/BL/6小鼠的左膝横向施加0.5 N的载荷，每天持续3分钟，连续3天。最后一次负荷后13天处死动物进行检查。对侧股骨作为非负荷对照，以p < 0.05评价负荷效应的统计学显著性。尽管测量到的骨干应变小至12 μ应变，但骨组织形态计量学清楚地表明骨形成的频率依赖性增强。与非负荷对照相比，骨膜表面的骨形成显著增强。15 Hz的载荷在提高矿化表面（1.7 x; p < 0.05）、矿物质沉积率（1.4 x; p < 0.001）和骨形成率（2.4 x; p < 0.01）方面最有效。在10 Hz下的负载提高了矿化表面（1.4 x; p < 0.05）、矿物质沉积率（1.3 x; p < 0.01）和骨形成率（1.8 x; p < 0.05）。在10 Hz（均为9%）和15 Hz（分别为12%和13%）的载荷下，股骨干中的皮质横截面积和皮质厚度显著增加。研究结果支持了膝关节负荷对股骨干皮质骨的合成代谢作用，并扩展了我们对骨与关节相互作用的认识。加强股骨有助于防止股骨骨折，并且关于所描述的膝关节载荷的发现可能提供一种新的策略来加强骨质疏松的骨。需要进一步的分析来理解膝关节负荷背后的生物物理和分子机制。
Bone alters its architecture and mass in response to the mechanical environment, and thus varying loading modalities have been examined for studying load-driven bone formation. The current study aimed to evaluate the anabolic effects of knee loading on diaphyseal cortical bone in the femur. Using a custom-made piezoelectric loader, 0.5-N loads were laterally applied to the left knee of C57/BL/6 mice at 5, 10, 15, and 20 Hz for 3 minutes per day for 3 consecutive days. Animals were sacrificed for examination 13 days after the last loading. The contralateral femur was used as a non-loading control, and the statistical significance of loading effects was evaluated with p < 0.05. Although diaphyseal strains were measured as small as 12 μstrains, bone histomorphometry clearly demonstrated frequency-dependent enhancement of bone formation. Compared to a non-loading control, bone formation on the periosteal surface was significantly enhanced. The loading at 15 Hz was most effective in elevating the mineralizing surface (1.7 x; p < 0.05), mineral apposition rate (1.4 x; p < 0.001), and bone formation rate (2.4 x; p < 0.01). The loading at 10 Hz elevated the mineralizing surface (1.4 x; p < 0.05), mineral apposition rate (1.3 x; p < 0.01), and bone formation rate (1.8 x; p < 0.05). The cross-sectional cortical area and the cortical thickness in the femoral diaphysis were significantly increased by loading at 10 Hz (both 9%) and 15 Hz (12% and 13%, respectively). The results support the anabolic effects of knee loading on diaphyseal cortical bone in the femur with small in situ strain, and they extend our knowledge on the interplay between bone and joints. Strengthening the femur contributes to preventing femoral fractures, and the discovery about the described knee loading might provide a novel strategy to strengthen osteoporotic bones. Further analyses are required to understand the biophysical and molecular mechanism behind knee loading.
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影响因子：
3.3
作者：
Turner, CH;Robling, AG
通讯作者：
Robling, AG
影响因子：
2.8
作者：
Pap, G;Eberhardt, R;Neumann, W
通讯作者：
Neumann, W
影响因子：
4.2
作者：
Flieger, J;Karachalios, T;Lyritis, G
通讯作者：
Lyritis, G
DOI：
10.2187/bss.18.41
发表时间：
2004-06-01
期刊：
Uchu Seibutsu Kagaku
影响因子：
--
作者：
Tanaka, Shigeo M;Sun, Hui B;Yokota, Hiroki
通讯作者：
Yokota, Hiroki
影响因子：
64.8
作者：
Rubin, C;Turner, AS;McLeod, K
通讯作者：
McLeod, K