Production of Citrate by Anaerobic Fungi in the Presence of Co-culture Methanogens as Revealed by (1)H NMR Spectrometry.
Production of Citrate by Anaerobic Fungi in the Presence of Co-culture Methanogens as Revealed by (1)H NMR Spectrometry.
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如（1）H NMR光谱法所示，在存在共培养甲烷基的情况下，通过厌氧菌真菌生产柠檬酸盐。
DOI：
10.5713/ajas.2013.13134
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发表时间：
2013-10
期刊：
影响因子：
--
通讯作者：
Zhu WY
中科院分区：
文献类型：
--
作者：
Cheng YF;Jin W;Mao SY;Zhu WY
厌氧真菌Piromyces sp. F1，从山羊的瘤胃中分离，以及如何这是受自然相关的产甲烷菌的存在下，代谢谱进行了分析，通过核磁共振光谱。在真菌单一培养中的主要代谢产物是甲酸盐、乳酸盐、乙醇、乙酸盐、琥珀酸盐、糖/氨基酸和α-酮戊二酸盐，而厌氧真菌和相关产甲烷菌的共培养产生柠檬酸盐。这是首次报道柠檬酸盐作为厌氧真菌的主要代谢产物。单因素分析表明，共培养条件下的甲酸、乳酸、乙醇、柠檬酸、琥珀酸和乙酸的平均值显著高于真菌单培养条件下的平均值，而葡萄糖和α-酮戊二酸的平均值显著降低。无监督的主成分分析揭示了真菌单培养物和共培养物的代谢产物谱的分离。总之，柠檬酸盐作为与产甲烷菌相关的厌氧真菌的主要代谢产物之一的新发现可能表明在共培养中存在一种新的尚待鉴定的途径。厌氧真菌代谢被相关的甲烷菌所改变，表明厌氧真菌是瘤胃中甲烷菌的重要底物提供者，因此在瘤胃甲烷生成中起关键作用。
The metabolomic profile of the anaerobic fungus Piromyces sp. F1, isolated from the rumen of goats, and how this is affected by the presence of naturally associated methanogens, was analyzed by nuclear magnetic resonance spectroscopy. The major metabolites in the fungal monoculture were formate, lactate, ethanol, acetate, succinate, sugars/amino acids and α-ketoglutarate, whereas the co-cultures of anaerobic fungi and associated methanogens produced citrate. This is the first report of citrate as a major metabolite of anaerobic fungi. Univariate analysis showed that the mean values of formate, lactate, ethanol, citrate, succinate and acetate in co-cultures were significantly higher than those in the fungal monoculture, while the mean values of glucose and α-ketoglutarate were significantly reduced in co-cultures. Unsupervised principal components analysis revealed separation of metabolite profiles of the fungal mono-culture and co-cultures. In conclusion, the novel finding of citrate as one of the major metabolites of anaerobic fungi associated with methanogens may suggest a new yet to be identified pathway exists in co-culture. Anaerobic fungal metabolism was shifted by associated methanogens, indicating that anaerobic fungi are important providers of substrates for methanogens in the rumen and thus play a key role in ruminal methanogenesis.
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影响因子：
4.4
作者：
BAUCHOP, T;MOUNTFORT, DO
通讯作者：
MOUNTFORT, DO
影响因子：
4.4
作者：
MARVINSIKKEMA, FD;RICHARDSON, AJ;PRINS, RA
通讯作者：
PRINS, RA
影响因子：
4.1
作者：
OFALLON, JV;WRIGHT, RW;CALZA, RE
通讯作者：
CALZA, RE
稳定同位素标记为动植物系统中的代谢物混合物的系统NMR分析：代谢途径的粗粒粒度。
影响因子：
3.7
作者：
Chikayama, Eisuke;Suto, Michitaka;Nishihara, Takashi;Shinozaki, Kazuo;Hirayama, Takashi;Kikuchi, Jun
通讯作者：
Kikuchi, Jun
影响因子：
2
作者：
Pham, LH;Vater, J;Mügge, C
通讯作者：
Mügge, C