In Vivo Characteristics of Premixed Calcium Phosphate Cements When Implanted in Subcutaneous Tissues and Periodontal Bone Defects.
In Vivo Characteristics of Premixed Calcium Phosphate Cements When Implanted in Subcutaneous Tissues and Periodontal Bone Defects.
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DOI：
10.6028/jres.115.021
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发表时间：
2010-07
影响因子：
1.5
通讯作者：
Chow LC
中科院分区：
文献类型：
--
作者：
Sugawara A;Fujikawa K;Hirayama S;Takagi S;Chow LC
先前的研究表明，无水预混磷酸钙骨水泥（Pre-CPC）比传统CPC具有更长的硬化时间和更低的强度，但在包装中稳定。材料仅在被输送到潮湿环境后硬化，并形成羟基磷灰石作为唯一的产物。当植入大鼠皮下组织时，前CPC也表现出良好的耐冲洗性和优异的生物相容性。本研究评价了前CPC植入皮下组织（研究I）和用于修复手术创建的双壁牙周缺损（研究II）时的特性。将CPC-1：Ca 4（PO 4）2 O和CaHPO 4、CPC-2：α-Ca 3（PO 4）2和CaCO 3或CPC-3：DCPA和Ca（OH）2组成的CPC粉末分别与甘油混合，以粉液质量比分别为3.5、2.5和2.5制备CPC前糊剂。在每种水泥混合物中，Ca与P的摩尔比为1.67。甘油含有Na 2 HPO 4（30质量%）和羟丙基甲基纤维素（0.55%），以分别促进水泥硬化和提高耐冲刷性。在研究I中，将试验材料皮下植入大鼠体内。术后4周处死动物，进行组织病理学观察。结果表明，所有植入材料在与植入物接触的组织中均表现出非常轻微或可忽略的炎症反应。在研究II中，成年比格犬的下颌前磨牙被拔除。一个月后，在下颌骨的牙齿附近通过手术创建双壁牙周骨缺损。用Pre-CPC糊剂填充缺损，并在术前位置更换皮瓣。分别于术后1、3、6个月处死动物，切除填充缺损的部分。结果显示，术后1个月，植入的Pre-CPC-1糊剂部分被骨替代，并在6个月时转化为骨。1个月内，填充有Pre-CPC-2的囊袋完全被新形成的骨覆盖。Pre-CPC-2在1个月内部分被松质骨取代，6个月内完全被骨取代。1个月和3个月样本的检查表明，前CPC-2吸收和被骨取代比前CPC 1更快。两种Pre-CPC糊剂均具有高度骨传导性。当植入牙周缺损中时，Pre-CPC-2比Pre-CPC-1更快地被骨替代。
Previous studies showed that water-free, premixed calcium phosphate cements (Pre-CPCs) exhibited longer hardening times and lower strengths than conventional CPCs, but were stable in the package. The materials hardened only after being delivered to a wet environment and formed hydroxyapatite as the only product. Pre-CPCs also demonstrated good washout resistance and excellent biocompatibility when implanted in subcutaneous tissues in rats. The present study evaluated characteristics of Pre-CPCs when implanted in subcutaneous tissues (Study I) and used for repairing surgically created two-wall periodontal defects (Study II). Pre-CPC pastes were prepared by combining CPC powders that consisted of CPC-1: Ca4(PO4)2O and CaHPO4, CPC-2: α-Ca3(PO4)2 and CaCO3 or CPC-3: DCPA and Ca(OH)2 with a glycerol at powder-to-liquid mass ratios of 3.5, 2.5, and 2.5, respectively. In each cement mixture, the Ca to P molar ratio was 1.67. The glycerol contained Na2HPO4 (30 mass %) and hydroxypropyl methylcellulose (0.55 %) to accelerate cement hardening and improve washout resistance, respectively. In Study I, the test materials were implanted subcutaneously in rats. Four weeks after the operation, the animals were sacrificed and histopathological observations were performed. The results showed that all of the implanted materials exhibited very slight or negligible inflammatory reactions in tissues contacted with the implants. In Study II, the mandibular premolar teeth of mature beagle dogs were extracted. One month later, two-wall periodontal bone defects were surgically created adjacent to the teeth of the mandibular bone. The defects were filled with the Pre-CPC pastes and the flaps replaced in the preoperative position. The dogs were sacrificed at 1, 3 and 6 months after surgery and sections of filled defects resected. Results showed that one month after surgery, the implanted Pre-CPC-1 paste was partially replaced by bone and was converted to bone at 6 months. The pockets filled with Pre-CPC-2 were completely covered by newly formed bone in 1 month. The Pre-CPC-2 was partially replaced by trabecular bone in 1 month and was completely replaced by bone in 6 months. Examination of 1 month and 3 month samples indicated that Pre-CPC-2 resorbed and was replaced by bone more rapidly than Pre-CPC 1. Both Pre-CPC pastes were highly osteoconductive. When implanted in periodontal defects, Pre-CPC-2 was replaced by bone more rapidly than Pre-CPC-1.
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DOI：
10.1002/jbm.b.10065
发表时间：
2003-11-15
影响因子：
3.4
作者：
Takagi, S;Chow, LC;Sugawara, A
通讯作者：
Sugawara, A
影响因子：
2.5
作者：
Fujikawa, K;Sugawara, A;Chow, LC
通讯作者：
Chow, LC
影响因子：
2.5
作者：
Sugawara, A;Fujikawa, K;Murai, S
通讯作者：
Murai, S
影响因子：
2
作者：
LOE, HARALD;SILNESS, JOHN
通讯作者：
SILNESS, JOHN
影响因子：
2.5
作者：
Sugawara, Akiyoshi;Fujikawa, Kenji;Chow, Laurence C.
通讯作者：
Chow, Laurence C.