Tea catechin auto-oxidation dimers are accumulated and retained by Caco-2 human intestinal cells.
Tea catechin auto-oxidation dimers are accumulated and retained by Caco-2 human intestinal cells.
复制标题
DOI：
10.1016/j.nutres.2010.05.006
复制
发表时间：
2010-05
期刊：
影响因子：
--
通讯作者：
Ferruzzi MG
中科院分区：
文献类型：
--
作者：
Neilson AP;Song BJ;Sapper TN;Bomser JA;Ferruzzi MG
尽管茶叶中存在生物活性的儿茶素B环自氧化二聚体，但人们对其在人体内的吸收知之甚少。我们对这项研究的假设是，儿茶素自氧化二聚体存在于茶叶中，并可被人类肠道上皮细胞吸收。用高效液相色谱-质谱法对市售茶叶中的二聚体[茶新素(THSNs)和P-2类似物]进行定量。表没食子儿茶素没食子酸酯(−)-表没食子儿茶素没食子酸酯(EGC)和(−)-表没食子儿茶素没食子酸酯(EGCG)同源二聚体的含量分别为10-43和0-62微克/克叶片。EGC-EGCG异源二聚体的存在范围为0-79微克分子/克。使用Caco-2肠道细胞来评估这些二聚体在肠道的潜在吸收。儿茶素单体和二聚体在暴露于含有单体和预制二聚体的培养液中的细胞中被检测到。二聚体的积累明显大于测试介质中的单体。EGC和EGCG的3h积累量分别为0.19-0.55%和1.24-1.35%。而EGC P-2类似物3h积累量和THSNs C/E分别为0.89±0.28%和1.53±0.36%。P-2、THSNs A/D比值分别为6.93±2.1%、10.1±3.6%。EGCG-EGC异源二聚体P-2类似物，Thsn B3h积累量分别为4.87±2.2%和4.65±2.8%。1h保留P-2，THSNs A/D为171±22%，占积累量的29.6±9.3%，提示THSNs在细胞内氧化转化为P-2。这些数据表明，肠腔中存在的儿茶素二聚体可能很容易被肠道上皮吸收。
Despite the presence of bioactive catechin B-ring auto-oxidation dimers in tea, little is known regarding their absorption in humans. Our hypothesis for this research is that catechin auto-oxidation dimers are present in teas and are absorbable by human intestinal epithelial cells. Dimers [theasinensins (THSNs) and P-2 analogs) were quantified in commercial teas by HPLC-MS. (−)-Epigallocatechin (EGC) and (−)-epigallocatechin gallate (EGCG) homodimers were present at 10–43 and 0–62 µmol/g leaf, respectively. EGC-EGCG heterodimers were present at 0–79 µmol/g. The potential intestinal absorption of these dimers was assessed using Caco-2 intestinal cells. Catechin monomers and dimers were detected in cells exposed to media containing monomers and preformed dimers. Accumulation of dimers was significantly greater than monomers from test media. Three h accumulation of EGC and EGCG was 0.19– 0.55% and 1.24–1.35% respectively. Comparatively, 3h accumulation of the EGC P-2 analog, and THSNs C/E was 0.89 ± 0.28% and 1.53 ± 0.36%. Accumulation of P-2, and THSNs A/D was 6.93 ± 2.1%, and 10.1 ± 3.6%. EGCG-EGC heterodimer P-2 analog, and THSN B 3h accumulation was 4.87 ± 2.2%, and 4.65 ± 2.8% respectively. One h retention of P-2, and THSNs A/D was 171 ± 22%, and 29.6 ± 9.3% of accumulated amount suggesting intracellular oxidative conversion of THSNs to P-2. These data suggest that catechin dimers present in the gut lumen may be readily absorbed by intestinal epithelium.
登录
查看更多内容
影响因子：
6.1
作者：
Neilson, Andrew P.;Hopf, Amber S.;Ferruzzi, Mario G.
通讯作者：
Ferruzzi, Mario G.
DOI：
10.1016/s0305-0491(97)00220-4
发表时间：
1997-11-01
影响因子：
2.2
作者：
Aw, TY
通讯作者：
Aw, TY
DOI：
10.1124/jpet.103.053496
发表时间：
2003-10-01
影响因子：
3.5
作者：
Hu, M;Chen, J;Lin, HM
通讯作者：
Lin, HM
影响因子：
8.1
作者：
Peters, Catrina M.;Green, Rodney J.;Janle, Elsa M.;Ferruzzi, Mario G.
通讯作者：
Ferruzzi, Mario G.
DOI：
10.1016/s1532-0456(02)00250-8
发表时间：
2003-03-01
影响因子：
3.9
作者：
Prabhu, R;Balasubramanian, KA
通讯作者：
Balasubramanian, KA