An inexpensive method of small paraffin tissue microarrays using mechanical pencil tips.
An inexpensive method of small paraffin tissue microarrays using mechanical pencil tips.
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DOI：
10.1186/1746-1596-6-117
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发表时间：
2011-12-01
影响因子：
2.6
通讯作者：
El-Hawary AK
中科院分区：
文献类型：
--
作者：
Shebl AM;Zalata KR;Amin MM;El-Hawary AK
组织微阵列技术提供了一种高通量的手段，评估档案病理标本中的潜在生物标志物。本研究的目的是使用机械铅笔尖生产组织微阵列块，而不需要高成本。使用常规的自动铅笔尖（Rotring Tikky II Mechanical Pills 1.0mm）从受体块切割出1mm蜡圆柱体，产生36至72个孔。通过使用机械铅笔尖从供体石蜡包埋的组织块手动冲出三个肿瘤区域的核心，并转移到石蜡组织微阵列的孔中。该技术简单，对供体块的损伤小。我们成功地进行了H&E载玻片和免疫检测，没有大量的组织圆柱体损失。我们的自动铅笔尖技术是文献中最便宜的简单技术。它还需要合理的时间，并减少免疫组织化学过程中的抗体消耗
Tissue microarray technology has provided a high throughput means of evaluating potential biomarkers in archival pathological specimens. This study was carried out in order to produce tissue microarray blocks using mechanical pencil tips without high cost. Conventional mechanical pencil tips (Rotring Tikky II Mechanical Pencil 1.0 mm) were used to cut out 1 mm wax cylinders from the recipient block, creating from 36 to 72 holes. Three cores of tumor areas were punched out manually by using the mechanical pencil tips from donor paraffin embedded tissue blocks and transferred to the holes of the paraffin tissue microarrays. This technique was easy and caused little damage to the donor blocks. We successfully performed H&E slides and immunodetection without substantial tissue cylinder loss. Our mechanical pencil tip technique is the most inexpensive easy technique among the literature. It also takes a reasonable amount of time and reduces antibody consumption during immunohistochemistry
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影响因子：
1
作者：
Singh, Deepak Kumar;Sakhuja, Puja;Gondal, Ranjana
通讯作者：
Gondal, Ranjana
影响因子：
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作者：
Hidalgo, A;Piña, P;Salcedo, M
通讯作者：
Salcedo, M
DOI：
10.1097/00129039-200306000-00015
发表时间：
2003-06-01
影响因子：
1.6
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通讯作者：
Liu, JS
影响因子：
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作者：
Brown, Lindsay A.;Huntsman, David
通讯作者：
Huntsman, David
DOI：
10.1097/00129039-200503000-00016
发表时间：
2005-03-01
影响因子：
1.6
作者：
Datta, MW;Kahler, A;Kajdacsy-Balla, A
通讯作者：
Kajdacsy-Balla, A