Peste des petits ruminants virus tissue tropism and pathogenesis in sheep and goats following experimental infection.
Peste des petits ruminants virus tissue tropism and pathogenesis in sheep and goats following experimental infection.
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DOI：
10.1371/journal.pone.0087145
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发表时间：
2014
期刊：
影响因子：
3.7
通讯作者：
Babiuk S
中科院分区：
文献类型：
--
作者：
Truong T;Boshra H;Embury-Hyatt C;Nfon C;Gerdts V;Tikoo S;Babiuk LA;Kara P;Chetty T;Mather A;Wallace DB;Babiuk S
小反刍兽疫（PPR）是一种病毒性疾病，主要影响小型反刍动物，给发展中国家的畜牧业造成重大经济损失。它是撒哈拉和撒哈拉以南非洲、中东和印度次大陆的地方病。小反刍兽疫病毒（PPRV）的主要宿主是山羊和绵羊;然而，最近研究PPRV病理学、疾病进展和病毒血症的模型主要集中在山羊模型上。本研究采用定量时程研究评价了绵羊和山羊实验感染后PPR的组织嗜性和发病机制。感染PPRV强毒株后，绵羊和山羊都出现了PPR典型的临床体征和病变，尽管绵羊的临床疾病比山羊轻。用实时荧光定量PCR和免疫组织化学方法检测PPRV的组织嗜性。淋巴结、淋巴组织和消化道器官是病毒复制的主要部位。本研究结果为PPRV在绵羊和山羊中的致病性和组织嗜性的比较评估提供了模型。这些模型适用于建立必要的实验参数的疫苗的评价，以及进一步研究PPRV宿主相互作用。
Peste des petits ruminants (PPR) is a viral disease which primarily affects small ruminants, causing significant economic losses for the livestock industry in developing countries. It is endemic in Saharan and sub-Saharan Africa, the Middle East and the Indian sub-continent. The primary hosts for peste des petits ruminants virus (PPRV) are goats and sheep; however recent models studying the pathology, disease progression and viremia of PPRV have focused primarily on goat models. This study evaluates the tissue tropism and pathogenesis of PPR following experimental infection of sheep and goats using a quantitative time-course study. Upon infection with a virulent strain of PPRV, both sheep and goats developed clinical signs and lesions typical of PPR, although sheep displayed milder clinical disease compared to goats. Tissue tropism of PPRV was evaluated by real-time RT-PCR and immunohistochemistry. Lymph nodes, lymphoid tissue and digestive tract organs were the predominant sites of virus replication. The results presented in this study provide models for the comparative evaluation of PPRV pathogenesis and tissue tropism in both sheep and goats. These models are suitable for the establishment of experimental parameters necessary for the evaluation of vaccines, as well as further studies into PPRV-host interactions.
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影响因子：
5.4
作者：
Berhe, G;Minet, C;Diallo, A
通讯作者：
Diallo, A
影响因子：
5.5
作者：
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影响因子：
3.3
作者：
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影响因子：
3.8
作者：
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通讯作者：
Boyle, David B.
影响因子：
2.2
作者：
Ayari-Fakhfakh, Emna;Ghram, Abdeljelil;Cetre-Sossah, Catherine
通讯作者：
Cetre-Sossah, Catherine