The Ph-3 gene from Solanum pimpinellifolium encodes CC-NBS-LRR protein conferring resistance to Phytophthora infestans.
The Ph-3 gene from Solanum pimpinellifolium encodes CC-NBS-LRR protein conferring resistance to Phytophthora infestans.
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来自 Solanum pimpinellifolium 的 Ph-3 基因编码 CC-NBS-LRR 蛋白，赋予对致病疫霉的抗性
DOI：
10.1007/s00122-014-2303-1
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发表时间：
2014-06
影响因子：
5.4
通讯作者：
Du, Yongchen
中科院分区：
文献类型：
--
作者：
Zhang, Chunzhi;Liu, Lei;Wang, Xiaoxuan;Vossen, Jack;Li, Guangcun;Li, Tao;Zheng, Zheng;Gao, Jianchang;Guo, Yanmei;Visser, Richard G. F.;Li, Junming;Bai, Yuling;Du, Yongchen
Ph-3是第一个克隆的番茄晚疫病抗性基因，编码CC-NBS-LRR蛋白。晚疫病是番茄上最具毁灭性的病害之一。来源于苦皮茄（Solanum pimpinellifolium）L3708的抗性（R）基因Ph-3提供了对多种致病疫霉分离物的抗性，并且已被广泛用于番茄育种计划。在我们以前的研究中，Ph-3被定位在第9号染色体长臂远端的一个含有R基因类似物（RGA）的区域。为了进一步缩小Ph-3区间，使用侧翼标记G2-4和M8-2鉴定了更多的重组体，根据Heinz 1706参考基因组，其将Ph-3基因限定为26 kb区域。为了克隆Ph-3基因，利用L3708构建了细菌人工染色体（BAC）文库，并鉴定了一个含有Ph-3区域的BAC克隆B25 E21。BAC克隆B25 E21的序列显示在靶区域中仅存在一个RGA。随后的互补分析表明，这种RGA，编码CC-NBS-LRR蛋白，能够补充品种Moneymaker的易感表型。因此，该RGA被认为是Ph-3基因。预测的Ph-3蛋白与9号染色体来源的马铃薯抗晚疫病菌抗性蛋白（Rpi蛋白）具有高度的氨基酸同一性。
Ph-3 is the first cloned tomato gene for resistance to late blight and encodes a CC-NBS-LRR protein. Late blight, caused by Phytophthora infestans, is one of the most destructive diseases in tomato. The resistance (R) gene Ph-3, derived from Solanum pimpinellifolium L3708, provides resistance to multiple P. infestans isolates and has been widely used in tomato breeding programmes. In our previous study, Ph-3 was mapped into a region harbouring R gene analogues (RGA) at the distal part of long arm of chromosome 9. To further narrow down the Ph-3 interval, more recombinants were identified using the flanking markers G2-4 and M8-2, which defined the Ph-3 gene to a 26 kb region according to the Heinz1706 reference genome. To clone the Ph-3 gene, a bacterial artificial chromosome (BAC) library was constructed using L3708 and one BAC clone B25E21 containing the Ph-3 region was identified. The sequence of the BAC clone B25E21 showed that only one RGA was present in the target region. A subsequent complementation analysis demonstrated that this RGA, encoding a CC-NBS-LRR protein, was able to complement the susceptible phenotype in cultivar Moneymaker. Thus this RGA was considered the Ph-3 gene. The predicted Ph-3 protein shares high amino acid identity with the chromosome-9-derived potato resistance proteins against P. infestans (Rpi proteins).
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影响因子：
5.4
作者：
Golas, Tomasz Michal;Sikkema, Anne;Gros, Jack;Feron, Richard M. C.;van den Berg, Ronald G.;van der Weerden, Gerard M.;Mariani, Celestina;Allefs, J. J. H. M.
通讯作者：
Allefs, J. J. H. M.
影响因子：
7.2
作者：
Huang, SW;van der Vossen, EAG;Visser, RGF
通讯作者：
Visser, RGF
DOI：
10.1080/14620316.2002.11511493
发表时间：
2002-05-01
影响因子：
1.9
作者：
Chunwongse, J;Chunwongse, C;Hanson, P
通讯作者：
Hanson, P
影响因子：
3.7
作者：
Huibers RP;Loonen AE;Gao D;Van den Ackerveken G;Visser RG;Bai Y
通讯作者：
Bai Y
影响因子：
3.5
作者：
Foster, Simon J.;Park, Tae-Ho;Jones, Jonathan D. G.
通讯作者：
Jones, Jonathan D. G.