Optical ATP biosensor for extracellular ATP measurement.
Optical ATP biosensor for extracellular ATP measurement.
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DOI：
10.1016/j.bios.2012.12.027
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发表时间：
2013-05-15
影响因子：
12.6
通讯作者：
Lin, W-C
中科院分区：
文献类型：
--
作者：
Wang, C.;Huang, C. -Y. C.;Lin, W-C
细胞外三磷酸腺苷（Adenosine-5′-triphosphate，ATP）是一种重要的多功能分子，通过激活嘌呤能P2受体介导多种生理活动。本研究的目的是开发一种新型的光学ATP传感器，用于生物组织中细胞外ATP的原位测量。通过在光纤探针末端涂覆两层溶胶-凝胶涂层，制成了光学ATP传感器。第一层含有钌络合物，用于感测由甘油激酶和甘油3-磷酸氧化酶氧化ATP引起的氧浓度变化，所述甘油激酶和甘油3-磷酸氧化酶包埋在第二层中。结果表明，光学ATP传感器能够在10 - 3 mM至1.5 mM的宽范围内检测ATP浓度。建立了补偿方法，使光学传感器能够在不同氧水平下测定ATP浓度。该研究还证明了ATP传感器测量生物组织中细胞外ATP含量的能力（即，猪椎间盘）。此外，它表明，光学ATP传感器不受pH和胞外ATP的衍生物。因此，新开发的光学ATP传感器是一个很好的选择，在原位细胞外ATP测量。
Extracellular Adenosine-5′-triphosphate (ATP) is an important multi-functional molecule which can mediate numerous physiological activities by activating purinergic P2 receptors. The objective of this study was to develop a novel optical ATP sensor for in-situ extracellular ATP measurement in biological tissues. The optical ATP sensor was made by applying two layers of sol-gel coating to the end of an optical fiber probe end. The first layer contained ruthenium complex for sensing changes in oxygen concentration which resulted from oxidation of ATP by glycerol kinase and glycerol 3-phosphate oxidase entrapped in the second layer. It was demonstrated that the optical ATP sensor was capable of detecting ATP concentration at a broad range of 10−3 mM to 1.5 mM. A compensation method was established to enable the optical sensor to determine ATP concentration at different oxygen levels. This study also demonstrated the capability of ATP sensor to measure extracellular ATP content in biological tissues (i.e., porcine intervertebral disc). In addition, it was shown that the optical ATP sensor was not affected by pH and derivatives of extracellular ATP. Therefore, the newly developed optical ATP sensor is a good option for in-situ extracellular ATP measurement.
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影响因子：
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影响因子：
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