Relationship Between Human Meibum Lipid Composition and the Severity of Meibomian Gland Dysfunction: A Spectroscopic Analysis.
Relationship Between Human Meibum Lipid Composition and the Severity of Meibomian Gland Dysfunction: A Spectroscopic Analysis.
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DOI：
10.1167/iovs.64.10.22
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发表时间：
2023-07-03
影响因子：
4.4
通讯作者：
中科院分区：
文献类型：
--
作者：
关于脂质组成与睑板腺功能障碍（MGD）严重程度之间关系的信息有限。本研究的目的是分析无MGD、轻度至中度MGD和重度MGD患者代谢产物的分子成分。有和没有MGD的成年人入组了一项前瞻性、多中心、探索性临床试验（ClinicalTrials.gov标识符：NCT01979887）。用1H-NMR测定了油脂样品中胆固醇酯与蜡酯的摩尔比（RCE/WE）和醛与蜡酯的摩尔比（Rald/WE）。根据最大代谢质量评分、Schirmer测试结果和受试者眼部症状问卷回答，对参与者的MGD疾病状态和严重程度（非MGD、轻至中度MGD和严重MGD）进行分组。来自69个个体的69个代谢样本被纳入分析：24个非MGD， 24个轻度至中度MGD， 21个严重MGD。非MGD的平均RCE/WE为0.29，轻至中度MGD的平均RCE/WE为0.14 (P = 0.038，比非MGD低51%)，严重MGD的平均RCE/WE为0.07 （P = 0.16，比轻至中度MGD低52%；P = 0.002，比非MGD低76%）。非MGD的平均Rald/WE为0.00022，轻至中度MGD的平均Rald/WE为0.00083 (P = 0.07，比非MGD高277%)，严重MGD的平均Rald/WE为0.0024 （P = 0.003，比轻至中度MGD高190%；P < 0.001，比非MGD高992%）。严重MGD组RCE/WE最低，Rald/WE最高，这表明这些细胞脂成分的摩尔比可能是由MGD相关的病理生理引起的，并可能影响眼表面脂质和泪膜的稳态。这些发现可能有助于确定MGD治疗的靶点。
Information on the relationship between meibum lipid composition and severity of meibomian gland dysfunction (MGD) is limited. The purpose of this study was to analyze the molecular components of meibum collected from individuals with no MGD, mild-to-moderate MGD, and severe MGD. Adults with and without MGD were enrolled in a prospective, multicenter, exploratory clinical trial (ClinicalTrials.gov Identifier: NCT01979887). Molar ratios of cholesteryl ester to wax ester (RCE/WE) and aldehyde to wax ester (Rald/WE) in meibum samples were measured with 1H-NMR spectroscopy. Results were evaluated for participants grouped by MGD disease status and severity (non-MGD, mild-to-moderate MGD, and severe MGD), as defined by maximum meibum quality scores, Schirmer test results, and Subject Ocular Symptom Questionnaire responses. Sixty-nine meibum samples from 69 individuals were included in the analysis: 24 non-MGD, 24 mild-to-moderate MGD, and 21 severe MGD. Mean RCE/WE was 0.29 in non-MGD, 0.14 in mild-to-moderate MGD (P = 0.038 vs. non-MGD, 51% lower), and 0.07 in severe MGD (P = 0.16 vs. mild-to-moderate MGD, 52% lower; P = 0.002 vs. non-MGD, 76% lower). Mean Rald/WE was 0.00022 in non-MGD, 0.00083 in mild-to-moderate MGD (P = 0.07 vs. non-MGD, 277% higher), and 0.0024 in severe MGD (P = 0.003 vs. mild-to-moderate MGD, 190% higher; P < 0.001 vs. non-MGD, 992% higher). RCE/WE was lowest and Rald/WE was highest in the severe MGD cohort, suggesting that these meibum constituent molar ratios may result from the pathophysiology associated with MGD and can impact ocular surface lipid and tear film homeostasis. These findings may potentially help identify targets for MGD treatment.
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