Distal hypoxic stroke: a new mouse model of stroke with high throughput, low variability and a quantifiable functional deficit.
Distal hypoxic stroke: a new mouse model of stroke with high throughput, low variability and a quantifiable functional deficit.
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DOI：
10.1016/j.jneumeth.2012.03.003
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发表时间：
2012-05-30
影响因子：
3
通讯作者：
Buckwalter, Marion S.
中科院分区：
文献类型：
--
作者：
Doyle, Kristian P.;Fathali, Nancy;Siddiqui, Mohammad R.;Buckwalter, Marion S.
C57 BL/6 J是中风实验中最常用的小鼠品系，但该品系内Willis环的血管解剖结构极其多变，并且皮质具有广泛的侧支化。这导致了近端靶向大脑中动脉的中风模型的巨大变异性，并在远端靶向大脑中动脉的中风模型中赋予了对缺血的抵抗力。我们测试了一个假设，即通过将大脑中动脉远端闭塞与1小时缺氧相结合，我们可以产生一个大的损伤，导致低变异性的行为缺陷。我们发现，这种新的远端缺氧（DH）中风模型产生的病变体积为同侧半球的25%，延伸到运动皮层，并导致行为缺陷。它还具有非常清晰的边界，异常低的可变性，并且可以由单个外科医生每天对多达30只动物进行手术。此外，年轻成年动物的存活率为100%，该模型可以在老年动物上进行，并且治疗干预可以减少梗死体积。因此，DH中风是对现有中风模型的极好补充，并且可用于C57 BL/6 J小鼠中的临床前研究。
C57BL/6J are the most commonly used strain of mouse for stroke experiments but vascular anatomy of the Circle of Willis within this strain is extremely variable and the cortex has extensive collateralization. This causes large variability in stroke models that target the middle cerebral artery proximally and confers resistance to ischemia in those that target it distally. We tested the hypothesis that by combining distal middle cerebral artery occlusion with 1 hour of hypoxia, we could generate a large lesion that causes a behavioral deficit with low variability. We found that this new distal hypoxic (DH) model of stroke generates a lesion with a volume of 25% of the ipsilateral hemisphere, extends to the motor cortex and causes a behavioral deficit. It also has a very clear border, exceptionally low variability, and can be performed by a single surgeon on up to 30 animals a day. Moreover, survivability is 100% in young adult animals, the model can be performed on old animals, and therapeutic intervention can reduce infarct volume. Therefore DH stroke is an excellent complement to existing stroke models and could be used for preclinical studies in C57BL/6J mice.
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影响因子：
8.3
作者：
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通讯作者：
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影响因子：
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作者：
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DOI：
10.1097/00004647-199805000-00012
发表时间：
1998-05-01
影响因子：
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作者：
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通讯作者：
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影响因子：
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作者：
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