Ribosomal protein NtRPL17 interacts with kinesin-12 family protein NtKRP and functions in the regulation of embryo/seed size and radicle growth.
Ribosomal protein NtRPL17 interacts with kinesin-12 family protein NtKRP and functions in the regulation of embryo/seed size and radicle growth.
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核糖体蛋白 NtRPL17 与驱动蛋白 12 家族蛋白 NtKRP 相互作用，并在调节胚胎/种子大小和胚根生长中发挥作用
DOI：
10.1093/jxb/erx361
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发表时间：
2017-11-28
影响因子：
6.9
通讯作者：
Sun MX
中科院分区：
文献类型：
--
作者：
Tian S;Wu J;Liu Y;Huang X;Li F;Wang Z;Sun MX
NtRPL 17和驱动蛋白NtKRP之间的直接相互作用控制植物细胞周期进程，并揭示了核糖体蛋白在种子大小建立中的新的调节作用。我们以前报道过，一种新的马达蛋白属于驱动蛋白-12家族，NtKRP，在调节胚胎和种子大小的建立中发挥关键作用。然而，目前还不清楚NtKRP如何促进这一发展过程。在这里，我们报告，60 S核糖体蛋白NtRPL 17直接与NtKRP相互作用。NtRPL 17 RNAi品系的表型显示出显著的胚和种子大小减小。NtRPL 17沉默的胚/种子的结构观察揭示胚尺寸减小是由于细胞数量减少。在这些胚胎中，细胞分裂周期进程在G2/M转换时延迟。这些表型与NtKRP沉默的胚胎/种子中的表型相似，表明NtKRP和NtRPL 17在种子发育期间在相同的调控途径中作为伴侣发挥作用，并特异性地调节细胞周期进程以控制胚胎/种子大小。本研究揭示了NtRPL 17作为一种广泛分布的核糖体蛋白，在种子发育过程中起着重要作用，并为研究种子大小的调控提供了新的线索。NtKRP和NtRPL 17之间的相互作用及其在细胞周期控制中的共同功能的确认也表明该机制可能在植物和动物中是保守的。
Direct interaction between NtRPL17 and the kinesin NtKRP controls cell cycle progression in plants and reveals a novel regulatory role for ribosomal proteins in seed size establishment. We previously reported that a novel motor protein belonging to the kinesin-12 family, NtKRP, displays critical roles in regulating embryo and seed size establishment. However, it remains unknown exactly how NtKRP contributes to this developmental process. Here, we report that a 60S ribosomal protein NtRPL17 directly interacts with NtKRP. The phenotypes of NtRPL17 RNAi lines show notable embryo and seed size reduction. Structural observations of the NtRPL17-silenced embryos/seeds reveal that the embryo size reduction is due to a decrease in cell number. In these embryos, cell division cycle progression is delayed at the G2/M transition. These phenotypes are similar to that in NtKRP-silenced embryos/seeds, indicating that NtKRP and NtRPL17 function as partners in the same regulatory pathway during seed development and specifically regulate cell cycle progression to control embryo/seed size. This work reveals that NtRPL17, as a widely distributed ribosomal protein, plays a critical role in seed development and provides a new clue in the regulation of seed size. Confirmation of the interaction between NtKRP and NtRPL17 and their co-function in the control of the cell cycle also suggests that the mechanism might be conserved in both plants and animals.
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