Mangrove bacterial diversity and the impact of oil contamination revealed by pyrosequencing: bacterial proxies for oil pollution.
Mangrove bacterial diversity and the impact of oil contamination revealed by pyrosequencing: bacterial proxies for oil pollution.
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DOI：
10.1371/journal.pone.0016943
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发表时间：
2011-03-02
期刊：
影响因子：
3.7
通讯作者：
Peixoto RS
中科院分区：
文献类型：
--
作者：
dos Santos HF;Cury JC;do Carmo FL;dos Santos AL;Tiedje J;van Elsas JD;Rosado AS;Peixoto RS
红树林是热带和亚热带地区过渡的沿海生态系统，是具有重要生物意义和生产力的生态系统。尽管红树林具有巨大的生态和经济重要性，但它们往往位于人为影响很大的地区，暴露在污染物中，例如石油泄漏释放的污染物。进行了一个微观世界实验，模拟了先前原始红树林沉积物中的一次漏油事件。用直接焦磷酸测序法研究了漏油对现有微生物群落的影响。在原始的红树林沉积物中观察到了广泛的细菌多样性，即使在石油污染之后也是如此。仅在污染样品中检测到的不同的OTUS数量显著高于仅在非污染样品中检测到的OTUS数量。变形杆菌门，特别是伽马变形杆菌和三角洲变形杆菌，在模拟漏油前后很普遍。另一方面，当暴露在2%和5%的油中时，色目和哈利亚目的数量减少，这被认为是油类污染的敏感指标。另外三个属，海洋杆菌，海洋杆菌和环状杆菌在面对石油时增加了它们的流行率。这些群体可能是生物监测红树林环境中石油影响的目标。我们建议使用所选属的序列作为石油污染的代用品，使用定量聚合酶链式反应评估。对不同红树林系统中的这些属与当地石油水平的关系进行量化，将有助于评估红树林的扰动程度，这在现场监测中是有用的。考虑到红树林对许多其他环境的重要性，以及这些地区对石油泄漏的敏感性，这份手稿将会引起广泛的兴趣。
Mangroves are transitional coastal ecosystems in tropical and sub-tropical regions and represent biologically important and productive ecosystems. Despite their great ecological and economic importance, mangroves are often situated in areas of high anthropogenic influence, being exposed to pollutants, such as those released by oil spills. A microcosm experiment was conducted, which simulated an oil spill in previously pristine mangrove sediment. The effect of the oil spill on the extant microbial community was studied using direct pyrosequencing. Extensive bacterial diversity was observed in the pristine mangrove sediment, even after oil contamination. The number of different OTUs only detected in contaminated samples was significantly higher than the number of OTUs only detected in non-contaminated samples. The phylum Proteobacteria, in particular the classes Gammaproteobacteria and Deltaproteobacteria, were prevalent before and after the simulated oil spill. On the other hand, the order Chromatiales and the genus Haliea decreased upon exposure to 2 and 5% oil, these are proposed as sensitive indicators of oil contamination. Three other genera, Marinobacterium, Marinobacter and Cycloclasticus increased their prevalence when confronted with oil. These groups are possible targets for the biomonitoring of the impact of oil in mangrove settings. We suggest the use of sequences of the selected genera as proxies for oil pollution, using qPCR assessments. The quantification of these genera in distinct mangrove systems in relation to the local oil levels would permit the evaluation of the level of perturbance of mangroves, being useful in field monitoring. Considering the importance of mangroves to many other environments and the susceptibility of such areas to oil spills this manuscript will be of broad interest.
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影响因子：
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作者：
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通讯作者：
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DOI：
10.1099/ijs.0.017061-0
发表时间：
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