GhTCP19 Transcription Factor Regulates Corm Dormancy Release by Repressing GhNCED Expression in Gladiolus
GhTCP19 Transcription Factor Regulates Corm Dormancy Release by Repressing GhNCED Expression in Gladiolus
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GhTCP19 转录因子通过抑制唐菖蒲中的 GhNCED 表达来调节球茎休眠释放
DOI：
10.1093/pcp/pcy186
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发表时间：
2018-09
影响因子：
4.9
通讯作者：
Yi Mingfang
中科院分区：
文献类型：
--
作者：
Wu Jian;Wu Wenjing;Liang Jiahui;Jin Yujie;Gazzarrini Sonia;He Junna;Yi Mingfang
休眠是植物生物学中最鲜为人知的现象之一，但在农业/园艺育种中，芽/球茎休眠是一个重要的经济性状。在本研究中，我们从球茎休眠释放(CDR)的转录组数据库中克隆了ABA生物合成基因GhNCED，并对其在调控CDR中的负作用进行了研究。为了了解GhNCED的转录调控，进行了酵母单杂交筛选，GhTCP19被鉴定为直接调控GhNCED的表达。体内实验表明，GhTCP19对GhNCED的表达具有负性调节作用。GhTCP19由外源细胞分裂素(CKS)显著诱导，并在CDR过程中被诱导。GhTCP19在休眠球茎中的沉默延迟了CDR，导致GhNCED和ABA水平的高表达。同时，GhTCP19沉默的球茎内源CK的生物合成和信号转导受到抑制。综上所述，我们的结果表明，GhTCP19通过抑制ABA生物合成基因(GhNCED)的表达，促进CK生物合成(GhIPT)和信号转导(GhARR)以及诱导细胞周期蛋白基因的表达，是CDR过程的正向调节因子。本研究拓展了我们对由TCP家族成员介导的CDR的认识。
Dormancy is one of the least understood phenomena in plant biology; however, bud/corm dormancy is an important economic trait in agricultural/horticultural breeding. In this study, we isolated an ABA biosynthesis gene, GhNCED, from the transcriptome database of corm dormancy release (CDR), and characterized its negative role in regulating CDR. To understand transcriptional regulation of GhNCED, yeast one-hybrid screening was conducted and GhTCP19 was identified and shown to regulate GhNCED expression directly. An in planta assay showed that GhTCP19 negatively regulates GhNCED expression. GhTCP19 is dramatically induced by exogenous cytokinins (CKs) and is induced during CDR. Silencing of GhTCP19 in dormant cormels delayed CDR, resulting in higher expression of GhNCED and ABA levels. Meanwhile, endogenous CK biosynthesis and signaling were inhibited in GhTCP19-silenced cormels. Taken together, our results reveal that GhTCP19 is a positive regulator of the CDR process by repressing expression of an ABA biosynthesis gene (GhNCED), promoting CK biosynthesis (GhIPT) and signal transduction (GhARR) as well as inducing cyclin genes. This study expands our knowledge on CDR which is mediated by TCP family members.
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影响因子：
6.9
作者：
Rueda-Romero P;Barrero-Sicilia C;Gómez-Cadenas A;Carbonero P;Oñate-Sánchez L
通讯作者：
Oñate-Sánchez L
影响因子：
8.8
作者：
Pruneda-Paz JL;Breton G;Nagel DH;Kang SE;Bonaldi K;Doherty CJ;Ravelo S;Galli M;Ecker JR;Kay SA
通讯作者：
Kay SA
OsTCP19 通过调节 ABI4 介导的途径影响发育和非生物应激信号传导。
影响因子：
4.6
作者：
Mukhopadhyay P;Tyagi AK
通讯作者：
Tyagi AK
影响因子：
4.3
作者：
C. Turnbull;Myriam A. A. Raymond-Myriam-A.-A.-Raymond-49952195;I. Dodd;S. Morris
通讯作者：
C. Turnbull;Myriam A. A. Raymond-Myriam-A.-A.-Raymond-49952195;I. Dodd;S. Morris
影响因子：
6.2
作者：
Xiong-hui Zhong;Xueyuan Yuan;Ze Wu;M. A. Khan;Jin Chen;Xiao-xin Li;Benhe Gong;Yang Zhao;Jian W
通讯作者：
Xiong-hui Zhong;Xueyuan Yuan;Ze Wu;M. A. Khan;Jin Chen;Xiao-xin Li;Benhe Gong;Yang Zhao;Jian W