Changes in membrane fluidity associated with lymphocyte stimulation by succinyl-concanavalin A.
Changes in membrane fluidity associated with lymphocyte stimulation by succinyl-concanavalin A.
复制标题
与琥珀酰刀豆球蛋白 A 刺激淋巴细胞相关的膜流动性变化。
DOI:
10.1016/0005-2736(82)90109-2
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发表时间:
1982
期刊:
影响因子:
--
通讯作者:
Deutsch,C
中科院分区:
文献类型:
--
作者:
Cherenkevich,SN;Vanderkooi,JM;Deutsch,C
Time-resolved fluorescence anisotropy studies of human peripheral blood lymphocytes labeled with 1,6-diphenyl-1,3,5-hexatriene were carried out at temperatures between 4 and 38°C. For unstimulated freshly-isolated lymphocytes the calculated order parameters were found to be 0.62 at 4°C and 0.44 at 37°C. Mitogen-induced alterations in the order parameter were evident within minutes after addition of succinyl-concanavalin A to the cells, increasing to a value of 0.56 after 2 h at 37°C. Both stimulated and unstimulated cells show a decrease in fluorescence anisotropy over the next 2–3 days of culture and after the third day the time-resolved fluorescence anisotropy decay profiles of the two populations of cells were indistinguishable. Our results indicate that there are both short- and long-term changes in the membranes of the cell upon stimulation by mitogen.
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