Changes in membrane fluidity associated with lymphocyte stimulation by succinyl-concanavalin A.

Changes in membrane fluidity associated with lymphocyte stimulation by succinyl-concanavalin A.
复制标题

与琥珀酰刀豆球蛋白 A 刺激淋巴细胞相关的膜流动性变化。

DOI:
10.1016/0005-2736(82)90109-2
复制
发表时间:
1982
期刊:
Biochimica et biophysica acta
影响因子:
--
通讯作者:
Deutsch,C
Deutsch,C
中科院分区:
--
文献类型:
--
作者:
Cherenkevich,SN;Vanderkooi,JM;Deutsch,C

文献摘要

参考文献

被引文献

相似文献

在4 - 38°C的温度下,对1,6-二苯基-1,3,5-己三烯标记的人外周血淋巴细胞进行了时间分辨荧光各向异性研究。对于未受刺激的新鲜分离的淋巴细胞,发现计算的顺序参数在4°C下为0.62,在37°C下为0.44。在向细胞中加入琥珀酰伴刀豆球蛋白A后几分钟内,有丝分裂原诱导的有序参数的改变是明显的,在37°C下2小时后增加到0.56的值。在接下来的2-3天的培养中,刺激的和未刺激的细胞都显示出荧光各向异性的降低,并且在第三天之后,两个细胞群体的时间分辨荧光各向异性衰减曲线是不可区分的。我们的研究结果表明,有丝分裂原刺激后,细胞膜有短期和长期的变化。
Time-resolved fluorescence anisotropy studies of human peripheral blood lymphocytes labeled with 1,6-diphenyl-1,3,5-hexatriene were carried out at temperatures between 4 and 38°C. For unstimulated freshly-isolated lymphocytes the calculated order parameters were found to be 0.62 at 4°C and 0.44 at 37°C. Mitogen-induced alterations in the order parameter were evident within minutes after addition of succinyl-concanavalin A to the cells, increasing to a value of 0.56 after 2 h at 37°C. Both stimulated and unstimulated cells show a decrease in fluorescence anisotropy over the next 2–3 days of culture and after the third day the time-resolved fluorescence anisotropy decay profiles of the two populations of cells were indistinguishable. Our results indicate that there are both short- and long-term changes in the membranes of the cell upon stimulation by mitogen.
人类白细胞中的酯酶
DOI: 10.1177/21.1.1
发表时间: 1973
影响因子: 3.2
作者:
C. Li;L. Yam;K. Lam
通讯作者: K. Lam
细胞周期期间嵌入小鼠白血病 L 1210 细胞膜中的 1,6-二苯基-1,3,5-己三烯的荧光偏振
DOI: 10.1016/0014-5793(78)80170-7
发表时间: 1978
期刊: FEBS Letters
影响因子: 3.5
作者:
A. Obrénovitch;C. Sené;M. Negre;M. Monsigny
通讯作者: M. Monsigny
用纯化的质膜诱导溶细胞 T 淋巴细胞。
DOI: 10.4049/jimmunol.120.4.1114
发表时间: 1978
影响因子: 4.4
作者:
F. Lemonnier;T. Mescher;L. Sherman;S. Burakoff
通讯作者: S. Burakoff
DOI: 10.1080/00268977400101511
发表时间: 1974
期刊: Molecular Physics
影响因子: 1.7
作者:
G. R. Luckhurst;M. Setaka;C. Zannoni
通讯作者: C. Zannoni
刺激生长的淋巴细胞、成纤维细胞和肝细胞中膜脂的流动性增加
DOI: 10.1016/0014-5793(77)80228-7
发表时间: 1977
期刊: FEBS Letters
影响因子: 3.5
作者:
John G. Collard;A. de Wildt;E. Oomen;J. Smeekens;P. Emmelot;M. Inbar
通讯作者: M. Inbar