A Method for Large-scale Propagation of Economic Plant Cultivars Eliminating Labor-Intensive Tissue Handling

该方案的目的是根据分化状态、活力、内源活力和遗传稳定性，开发在液体培养愈伤组织再生可移植植株过程中进行组织等量转移的非破坏性自动化技术。他将根据分化组织和未分化组织的特性差异，测试实验室原型在非破坏性分离组织方面的有效性，并评估由此得出的系统的可靠性。他还将确定悬浮在透明介质中时要转移的组织的光学性质，并设计能够可靠地评估组织分离的光学或其他系统。这一建议解决了克隆植物繁殖的一个重要制约因素。它提出了一些方法，如果成功，将消除大规模繁殖植物组织培养过程中的劳动密集型阶段。

The aim of the proposal is to develop nondamaging automated techniques for transfer of tissue aliquots in the regeneration of transplantable plants from callus cultures in liquid culture on the basis of the state of differentiation, viability, endogenous vigor and genetic stability. He would test laboratory prototypes for efficacy in nondestructive separation of tissues on the basis of differences in the properties of differentiated and nondifferentiated tissues and assess the reliability of the systems thus derived. He would also determine the optical properties of the tissues to be transferred while suspended in a transparent medium and devise optical or other systems capable of reliably assessing tissue separation. This proposal addresses an important constraint upon clonal plant propagation. It proposes methods which, if successful, would eliminate the labor intensive stages in handling of plant tissue cultures for large scale propagation.