Cloning and Functional Analysis of Fungal Differentiation- Specific Genes and Intracellular Localization of the Gene Products

菜豆锈菌入侵寄主植物>单胞锈菌> > appropriulatus>，通过气孔发生，需要 感染结构的发育，例如附着胞。 附着胞形成是细胞分化事件， 发生在对接触（触变）刺激的反应中。 细胞 分化开始于核分裂的开始。 至少有15种蛋白质是人工合成的 当胚胎被诱导分化时， 被激活的基因的性质， 蛋白质的作用。 在上一个资助期内， 已经分离出了一些基因，这些基因是由 时间的差异是完全的。 斯台普斯博士现在将分析 基因的详细结构，时间， 当它们第一次表达时， 与其他真菌DNA同源性，以及鉴定 他们的基因产物。 他会准备针对 这些蛋白质基因产物，并使用它们来确定 蛋白质在胚中的位置。 此外 将尝试分离分化相关基因 在分化过程中早于6h表达 这是一个过程，即在分化完成之前。 这些研究将大大提高我们对 参与真菌感应反应的机制， 附着胞在气孔上的准确位置 在宿主殖民过程中。

Invasion of host plants by the bean rust fungus, >>>Uromyces> >appendiculatus>, occurs through stomata and requires the development of infection structures, e.g. appressoria. Appressorium formation is a cell differentiation event that occurs in response to contact (thigmotropic) stimuli. Cell differentiation begins with the onset of nuclear division. Evidence that at least fifteen species of protein are synthesized when germlings are induced to differentiate, has led to the study of the nature of the genes which are activated and the nature and role of the proteins. During the previous granting period four genes have been isolated that are specifically expressed by the time differentiation is complete. Dr. Staples will now analyze the dr.genes in terms of detailed structures, the time in the differentiation process when they are first expressed, their homology with DNA from other fungi, and an identification of their gene products. He will prepare antibodies specific for these protein gene products and use them to determine the location in the germling of the dr.proteins. In addition attempts will be made to isolate differentiation related genes that are expressed earlier than 6 h in the differentiation process, i.e. before differentiation is complete. The studies will significantly advance our knowledge of the mechanisms involved in the fungal sensing responses that result in the accurate placement of the appressorium over the stomate during host colonization.