Understanding why mammalian eggs have so much mitochondrial DNA

了解为什么哺乳动物的卵子有如此多的线粒体 DNA

• 批准号: DP230103127
• 负责人: Dr Deepak Adhikari
• 金额: $ 28.68万
• 依托单位: Monash University
• 依托单位国家: 澳大利亚
• 项目类别: Discovery Projects
• 财政年份: 2023
• 资助国家: 澳大利亚
• 起止时间: 2023-01-01 至 2025-12-31
• 项目状态: 未结题
• 来源: https://dataportal.arc.gov.au/RGS/Web/Grants/DP230103127
• 关键词: Understanding; why; mammalian; eggs; so

During oocyte growth there is massive increase in the replication of mitochondrial DNA so that each ovulated egg has 200,000-400,000 copies of the mitochondrial genome. This mitochondrial compliment will provide the template for all mitochondrial DNA in the subsequent organism. The established role of mitochondria is to provide energy in the form of ATP, but they are also known to be highly adaptive to the metabolic and energetic state of the cell. In this project, we will use genetic approaches to decrease the amount of oocyte mitochondrial DNA by 90%. We will examine how this influences mitochondrial organisation, oocyte metabolism and embryo development. This new knowledge will provide insights into animal breeding and human health.

在卵母细胞的生长过程中，线粒体DNA的复制大量增加，因此每个排卵的卵子都有200,000-400,000个线粒体基因组拷贝。这种线粒体互补将为后续生物体中的所有线粒体DNA提供模板。线粒体的既定作用是以ATP的形式提供能量，但众所周知，它们也高度适应细胞的新陈代谢和能量状态。在这个项目中，我们将使用遗传方法将卵母细胞线粒体DNA的数量减少90%。我们将研究这如何影响线粒体组织、卵母细胞新陈代谢和胚胎发育。这一新知识将为动物育种和人类健康提供见解。