Research Initiation Award: Investigation of the Effects of Dissolved Oxygen and Dissolved Carbon Dioxide on Embryogenesis and the Competence for Embryogenesis

叙述：胡萝卜的体细胞胚胎发生将被用作量化溶解氧和溶解二氧化碳对未分化细胞生长和胚胎发生的影响的模型。之所以选择这个模式，是因为有关胡萝卜体细胞胚胎发生的大量信息已经存在于文献中。胚胎被认为来自单个细胞，或与一簇细胞相关的少量细胞。在诱导后，胚胎经历了几个发育阶段。对胚胎发生和植株再生不同阶段的认识将构成评估该项目胚胎发生的主要分析程序。不同组成的气体将被用于摇瓶研究，然后信息将被转移到更适用于工业的搅拌、可扩展反应器系统。将研究氧气和二氧化碳的影响，以开发一种可以在受控水平的气体成分下运行的生物反应器。这一发展将为进一步的研究提供宝贵的工具，这些研究将需要在明确的营养条件下培养胚胎。

Narrative: The somatic embryogenesis of carrots will be used as a model to quantify the effects of dissolved oxygen and dissolved carbon dioxide on undifferentiated cell growth and embryogenesis. This model has been selected because extensive information regarding somatic embryogenesis of carrots already exists in the literature. Embryos are thought to originate from single cells, or a small number of cells associated with a cluster of cells. After induction, the embryos progress through several stages of development. Recognition of the various stages of embryogenesis and plantlet regeneration will comprise the major analytical procedures for assessing embryogenesis for this project. Gases of various composition will be used in shake flask studies and the information will then be transferred to the more industrially applicable system of an agitated, scalable reactor. The oxygen and carbon dioxide effects will be studied to develop a bioreactor that can be operated at controlled levels of the gaseous components. This development will provide a valuable tool for further investigations that will require the cultivation of embryos under well defined nutritional conditions.