DMREF: Thin Film Biofabrication for Integrated Bio-electronics

DMREF：集成生物电子学薄膜生物制造

• 批准号: 1435957
• 负责人: Gregory Payne
• 金额: $ 100.71万
• 依托单位: University of Maryland, College Park
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2014
• 资助国家: 美国
• 起止时间: 2014-09-01 至 2020-09-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1435957&HistoricalAwards=false
• 关键词: DMREF; Thin; Film; Biofabrication; Integrated

1435957University of Maryland College ParkPayneThe PIs propose to integrate advances in biotechnology and microelectronics by developing generic, biologically-based thin film fabrication methods to incorporate biological function into electronics. Thin hydrogel films will be triggered to self-assemble in response to locally-imposed electrical signals. The PIs propose methods to incorporate small molecules, proteins, and cells into the hydrogel films, and will focus on understanding how fabrication conditions control the structure-property-function relations. A multilayer thin film system is proposed that will allow communication between the biology and the electronics.The work is potentially transformative in four ways. First, the PIs will apply the advances in biology as an underpinning for materials science. Second, theory and experiments will be coupled to characterize the mesoscale self-assembly of soft matter. Third, the spatial, temporal and quantitative precision of electrical signals will be used to create structure, confer properties and perform functions. Finally, the biotechnology and electronics will be integrated.Strong international collaborations are proposed which will provide students with opportunities to participate in projects that span continents and cultures. The proposed collaboration with industrial partners will accelerate the incorporation of biology into materials science. The PIs also propose to integrate the activities within existing University partnerships with Food and Drug Administration and the National Institutes of Science and Technology. Because they provide the metrology and regulatory guidelines, these agencies will be integral to the speed that biologically-based materials are introduced into the marketplace. These partnerships will assist in accelerating translation and also in providing meaningful externships for students.

1435957 马里兰大学帕克分校佩恩分校 PI 提议通过开发通用的、基于生物的薄膜制造方法将生物功能融入电子学中，从而整合生物技术和微电子学的进步。 水凝胶薄膜将响应局部施加的电信号而被触发自组装。 PI 提出了将小分子、蛋白质和细胞整合到水凝胶薄膜中的方法，并将重点了解制造条件如何控制结构-性能-功能关系。提出了一种多层薄膜系统，该系统将允许生物学和电子学之间的通信。这项工作在四个方面具有潜在的变革性。首先，PI 将应用生物学的进步作为材料科学的基础。其次，将理论和实验结合起来表征软物质的介观自组装。第三，电信号的空间、时间和定量精度将用于创建结构、赋予特性和执行功能。最后，生物技术和电子学将被整合。强有力的国际合作将为学生提供参与跨越大陆和文化的项目的机会。 拟议与工业合作伙伴的合作将加速生物学与材料科学的结合。 PI 还建议将这些活动整合到大学与食品和药物管理局以及美国国家科学技术研究院现有的合作伙伴关系中。由于它们提供计量和监管指南，这些机构将成为生物基材料进入市场速度不可或缺的一部分。这些合作伙伴关系将有助于加快翻译速度，并为学生提供有意义的实习机会。

项目成果

A redox-based electrogenetic CRISPR system to connect with and control biological information networks

• DOI: 10.1038/s41467-020-16249-x
• 发表时间: 2020-05-15
• 期刊: NATURE COMMUNICATIONS
• 影响因子: 16.6
• 作者: Bhokisham, Narendranath;VanArsdale, Eric;Bentley, William E.
• 通讯作者: Bentley, William E.

Mediated Electrochemistry to Mimic Biology's Oxidative Assembly of Functional Matrices

• DOI: 10.1002/adfm.202001776
• 发表时间: 2020-06-02
• 期刊: ADVANCED FUNCTIONAL MATERIALS
• 影响因子: 19
• 作者: Li, Jinyang;Kim, Eunkyoung;Payne, Gregory F.
• 通讯作者: Payne, Gregory F.

Plasmid‐encoded protein attenuates Escherichia coli swimming velocity and cell growth, not reprogrammed regulatory functions

质粒编码的蛋白质减弱了大肠杆菌的游动速度和细胞生长，而不是重新编程的调节功能

• DOI: 10.1002/btpr.2778
• 发表时间: 2019
• 期刊: Biotechnology Progress
• 影响因子: 2.9
• 作者: Virgile, Chelsea;Hauk, Pricila;Wu, Hsuan‐Chen;Bentley, William E.
• 通讯作者: Bentley, William E.

Coupling Self-Assembly Mechanisms to Fabricate Molecularly and Electrically Responsive Films

耦合自组装机制来制造分子和电响应薄膜

• DOI: 10.1021/acs.biomac.8b01592
• 发表时间: 2019-02-01
• 期刊: BIOMACROMOLECULES
• 影响因子: 6.2
• 作者: Li, Jinyang;Maniar, Drishti;Payne, Gregory F.
• 通讯作者: Payne, Gregory F.

Bacterial co-culture with cell signaling translator and growth controller modules for autonomously regulated culture composition

• DOI: 10.1038/s41467-019-12027-6
• 发表时间: 2019-09-11
• 期刊: NATURE COMMUNICATIONS
• 影响因子: 16.6
• 作者: Stephens, Kristina;Pozo, Maria;Bentley, William E.
• 通讯作者: Bentley, William E.