Award For Creativity in Engineering for Kimberlee Wallace

次级代谢物是由代谢产物产生的化合物。 但不是细胞生长所必需的。的实例 商业上重要的次级代谢物包括 抗生素色素和香料由于知识有限， 这些化合物产生的复杂途径， 提高生产率的传统方法包括 经验技术。在早期，这些经验策略 导致生产力指数增长;然而，目前， 这些技术不太成功，导致每年 生产率只提高了几个百分点。获得更大 为了提高生产率，必须开发合理的技术 基于细胞内速率限制的知识， 生物合成 %%% 本研究的目的是检测细胞内 环境，并确定对辅助设备的速率限制 代谢产物的产生。调查员建议检查 三个层面的监管。首先，一种关键途径酶的水平 将被量化，以确定酶的可用性是否是一个 限制生物合成。其次，前体供应将是 检查以确定代谢物是否通过 #21453;路限制了生产。三、反作用效果 将检查生物合成的终产物，以确定是否 细胞内水平抑制产生。

Secondary metabolites are compounds which are produced by the cell but are not required for the growth of the cell. Examples of commercially important secondary metabolites include the antibiotics, pigments, and fragrances. Due to the limited knowledge of the complex pathways from which these compounds are produced, traditional methods for improving productivity have involved empirical techniques. In early years, these empirical strategies resulted in exponential productivity increases; however, currently these techniques are less successful, resulting in annual productivity increases of only a few percent. To obtain greater productivity improvements, rational techniques must be developed based upon knowledge of intracellular rate limitations to biosynthesis. %%% The purpose of this study is to examine the intracellular environment and determine the rate limitation to secondary metabolite production. The investigator proposes to examine regulation at three levels. First, levels of a key pathway enzyme will be quantified to determine if enzyme availability is a limitation to biosynthesis. Second, precursor supplies will be examined to determine if the flux of metabolites through the pathway limits production. Third, the effect of reaction endproducts on biosynthesis will be examined to determine if intracellular levels inhibit production.