Combinatorial Approach to Enzymatic Polymer Processing

酶促聚合物加工的组合方法

• 批准号: 0114790
• 负责人: Gregory Payne
• 金额: $ 41.56万
• 依托单位: University of Maryland Biotechnology Institute
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2001
• 资助国家: 美国
• 起止时间: 2001-09-01 至 2005-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0114790&HistoricalAwards=false
• 关键词: Combinatorial; Approach; Enzymatic; Polymer; Processing

The aim is to emulate the reaction processes used in nature for lignin polymerization, the setting of mussel glue, and insect shell hardening. These polymerization and curing processes are enzyme-initiated and then they progress in a "domino" fashion. In this project, it is proposed to use tyrosinase to convert natural phenolic precursors into reactive o-quinones, which can then serve as agents for polysaccharide crosslinking and protein-polysaccharide coupling. To elucidate more mechanistic details, a combined biochemical and mechanical screening program has been devised.

其目的是模拟自然界中用于木质素聚合、贻贝胶的凝固和昆虫壳硬化的反应过程。 这些聚合和固化过程是酶引发的，然后它们以“多米诺骨牌”方式进行。 在这个项目中，提出使用酪氨酸酶将天然酚类前体转化为反应性邻醌，然后可以作为多糖交联和蛋白质-多糖偶联的试剂。 为了阐明更多的机制细节，结合生物化学和机械筛选程序已经设计出来。