Combinatorial Approach to Enzymatic Polymer Processing

其目的是模拟自然界中用于木质素聚合、贻贝胶的凝固和昆虫壳硬化的反应过程。 这些聚合和固化过程是酶引发的，然后它们以“多米诺骨牌”方式进行。 在这个项目中，提出使用酪氨酸酶将天然酚类前体转化为反应性邻醌，然后可以作为多糖交联和蛋白质-多糖偶联的试剂。 为了阐明更多的机制细节，结合生物化学和机械筛选程序已经设计出来。

The aim is to emulate the reaction processes used in nature for lignin polymerization, the setting of mussel glue, and insect shell hardening. These polymerization and curing processes are enzyme-initiated and then they progress in a "domino" fashion. In this project, it is proposed to use tyrosinase to convert natural phenolic precursors into reactive o-quinones, which can then serve as agents for polysaccharide crosslinking and protein-polysaccharide coupling. To elucidate more mechanistic details, a combined biochemical and mechanical screening program has been devised.