Developing Polymeric Separating Agents to Mimic the Specificity of Protein Binding

开发聚合物分离剂来模拟蛋白质结合的特异性

• 批准号: 8912141
• 负责人: Gregory Payne
• 金额: $ 9.18万
• 依托单位: University of Maryland Baltimore County
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1990
• 资助国家: 美国
• 起止时间: 1990-02-15 至 1993-01-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=8912141&HistoricalAwards=false
• 关键词: Developing; Polymeric; Separating; Agents; Mimic

The proposed work is an initial step toward developing synthetic polymeric sorbents that mimic biological binding mechanisms. Proteins exhibiting multi-interactive mechanisms and competition at a particular site are selectively satisfied by those protein materials which most closely fit energetically a specific binding point. The research will initially demonstrate a separating agent capable of selectively absorbing solutes that require mechanisms which utilize both hydrogen bonding and hydrophobic interaction. To develop improved separating agents by mimicing nature requires a thorough understanding of relevant mechanistic interactions. Although a large amount of information is being generated on how proteins bind to molecules, there is considerably less knowledge concerning solid-liquid adsorption mechanisms. Thus, it is proposed to initially examine three binding mechanisms; hydrogen bonding, hydrophobic interactions and charge transfer complexation. Finally, in the later stages of the work it follows that these interactions will be coupled to develop separating agents which mimic biological binding. To provide a better understanding of these phenomena as well as to permit the extrapolation of these results, appropriate thermodynamic descriptions have been developed.

拟议的工作是朝着发展的第一步 模拟生物结合的合成聚合物吸附剂 机制等 表现出多重相互作用机制的蛋白质 和竞争在一个特定的网站是有选择地满足 这些蛋白质材料最适合 是一个特殊的结合点。 这项研究将 初步证明分离剂能够 选择性地吸收溶质，需要机制， 利用氢键和疏水相互作用。 通过模仿自然来开发改进的分离剂 需要彻底了解相关的机械 交互. 虽然大量的信息被 产生于蛋白质如何与分子结合， 关于固液吸附的知识相当少 机制等 因此，建议初步审查三个 结合机制;氢键，疏水相互作用 和电荷转移络合。 最后，在后来的 工作的各个阶段，这些相互作用将是 再加上开发分离剂， 约束力 为了更好地理解这些现象 以及允许这些结果的外推， 已经开发了适当的热力学描述。