Acquisition of an Integrated System for Combined Quantitative Microscopic Fluorescence Analysis and Electrophysiological Recording

本提案请求支持购买几台设备，以建立一个结合定量显微荧光分析和电生理记录的集成系统。六个主要用户已经提出了将使用该仪器的项目。其中五项涉及钙离子的荧光测量与膜运输或受体研究（肥大细胞的胞外分泌，哺乳动物神经元的受体研究，肠电解质运输，尼古丁受体和Ca2+通道功能，黄体细胞中的Ca2+运动和激素产生）。第六个项目将使用Ca2+介导的荧光来研究培养中的肌管。

This proposal requests support to obtain several pieces of equipment to establish an integrated system for combined quantitative microscopic fluorescence analysis and electrophysiological recording. Six major users have proposed projects that will utilize the instrumentation. Five of these involve Ca2+ measurements by fluorescence coupled to membrane transport or receptor studies (exocytosis in Mast cells, receptor studies in mammalian neurons, intestinal electrolyte transport, nicotinic receptors and Ca2+channel function, Ca2+ movements and hormone production in luteal cells). The sixth project will use Ca2+-mediated fluorescence to study muscle myotubes in culture.