Acquisition of an Integrated System for Combined Quantitative Microscopic Fluorescence Analysis and Electrophysiological Recording

获取用于组合定量显微荧光分析和电生理记录的集成系统

• 批准号: 8716854
• 负责人: Linda Nowak
• 金额: --
• 依托单位: Cornell University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1988
• 资助国家: 美国
• 起止时间: 1988-04-15 至 1990-09-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=8716854&HistoricalAwards=false
• 关键词: Acquisition; Integrated; System; Combined; Quantitative

This proposal requests support to obtain several pieces of equipment to establish an integrated system for combined quantitative microscopic fluorescence analysis and electrophysiological recording. Six major users have proposed projects that will utilize the instrumentation. Five of these involve Ca2+ measurements by fluorescence coupled to membrane transport or receptor studies (exocytosis in Mast cells, receptor studies in mammalian neurons, intestinal electrolyte transport, nicotinic receptors and Ca2+channel function, Ca2+ movements and hormone production in luteal cells). The sixth project will use Ca2+-mediated fluorescence to study muscle myotubes in culture.

该提案要求获得支持，以获得几台设备，以建立一个将定量显微荧光分析和电生理记录相结合的综合系统。六个主要用户已经提出了将利用该仪器的项目。其中五项涉及结合膜转运或受体研究的荧光测量钙离子(肥大细胞的胞吐作用、哺乳动物神经元的受体研究、肠道电解质转运、尼古丁受体和钙通道功能、钙离子运动和黄体细胞的激素产生)。第六个项目将使用钙离子介导的荧光来研究培养中的肌肉肌管。