Acquisition of an Integrated System for Combined Quantitative Microscopic Fluorescence Analysis and Electrophysiological Recording

该提案要求获得支持， 设备建立一个综合系统， 定量显微荧光分析， 电生理记录 六大用户提出 将使用仪器的项目。 其中五 包括通过与膜耦合的荧光测量Ca2+ 转运或受体研究（肥大细胞胞吐，受体 哺乳动物神经元研究，肠电解质转运， 烟碱受体和Ca2+通道功能，Ca2+运动， 黄体细胞中的激素产生）。 第六个项目将使用 Ca2+介导的荧光研究培养中的肌管。

This proposal requests support to obtain several pieces of equipment to establish an integrated system for combined quantitative microscopic fluorescence analysis and electrophysiological recording. Six major users have proposed projects that will utilize the instrumentation. Five of these involve Ca2+ measurements by fluorescence coupled to membrane transport or receptor studies (exocytosis in Mast cells, receptor studies in mammalian neurons, intestinal electrolyte transport, nicotinic receptors and Ca2+channel function, Ca2+ movements and hormone production in luteal cells). The sixth project will use Ca2+-mediated fluorescence to study muscle myotubes in culture.