Molecular Analysis of the Viviparous-l Gene in Maize

玉米 Viviparous-l 基因的分子分析

• 批准号: 8718374
• 负责人: Donald McCarty
• 金额: $ 23.84万
• 依托单位: University of Florida
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 1988
• 资助国家: 美国
• 起止时间: 1988-06-01 至 1992-05-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=8718374&HistoricalAwards=false
• 关键词: Molecular; Analysis; Viviparous; Gene; Maize

The viviparous-1 (vp) gene of maize blocks the normal arrest of embryo development, thereby causing precocious germination of the seed on the mother plant. The objectives of this proposal are to 1) isolate the vp gene by transposon tagging and determine its molecular structure and 2) identify the vp gene product by making antibody to a synthetic peptide derived from the gene sequence. Dr. McCarty's goal to clone the vp gene is very important for the study of developmental gene regulation and seed development in maize. The findings generated by this work will clearly set the stage for significant future work.

玉米的胎生1号(Vp)基因阻止了胚胎发育的正常停止，从而导致母本种子的早熟萌发。本研究的目的是：1)利用转座子标签技术分离VP基因并确定其分子结构；2)通过对基因序列合成的多肽产生抗体来鉴定VP基因产物。McCarty博士的目标是克隆VP基因，这对研究玉米发育基因调控和种子发育具有重要意义。这项工作产生的结果将清楚地为今后的重大工作奠定基础。