Functional Analysis of B3 Domain Genes in Maize and Arabidopsis

玉米和拟南芥B3结构域基因的功能分析

• 批准号: 0080175
• 负责人: Donald McCarty
• 金额: $ 36万
• 依托单位: University of Florida
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2000
• 资助国家: 美国
• 起止时间: 2000-08-01 至 2003-07-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0080175&HistoricalAwards=false
• 关键词: Functional; Analysis; B3; Domain; Genes

0080175McCartyDr. McCarty will continue his analysis of genes in maize and arabidopsis that encode B3 type DNA binding proteins. He has shown that the B3 protein structure defines a new class of DNA binding proteins that are found only in the genomes of plants. In plants, B3 genes include a family of regulatory genes that control key aspects of seed development, hormone signaling, and leaf and floral organ development. He is intrigued by the broader issue of the role kingdom specific transcription factors like the B3 proteins have had in the evolution of eukaryotic organisms. He will dissect the developmental functions of key B3 gene families in two distantly related plant species, maize and arabidopsis. He will leverage a robust set of molecular tools and new insight into how B3 proteins are regulated. He will characterize a regulatory mutation in B3 and use that mutant as a basis for mutant screens in arabidopsis. He will extend our use of the aleurone cell layer of the maize endosperm as a genetic model for analysis B3 function in monocot plant development. Finally, he will begin functional analysis of a second B3 gene family that reveals intriguing qualitative differences in monocots and dicots.

0080175 mccartydr。麦卡蒂将继续分析玉米和拟南芥中编码B3型DNA结合蛋白的基因。他已经证明B3蛋白结构定义了一类新的DNA结合蛋白，这种蛋白只存在于植物基因组中。在植物中，B3基因包括一个调控基因家族，控制种子发育、激素信号、叶和花器官发育的关键方面。他对像B3蛋白这样的王国特异性转录因子在真核生物进化中所起的作用这一更广泛的问题很感兴趣。他将分析两个远亲植物物种——玉米和拟南芥中关键B3基因家族的发育功能。他将利用一套强大的分子工具和对B3蛋白如何调节的新见解。他将描述B3中的一个调控突变，并将该突变作为拟南芥突变筛选的基础。他将扩展我们对玉米胚乳糊粉细胞层的使用，作为分析单子叶植物发育中B3功能的遗传模型。最后，他将开始对第二个B3基因家族进行功能分析，揭示单子房和双子房的有趣的定性差异。